Study of cultural properties and biological activity Mycobacterium avium IEKVM-UAAS

  • A. I. Sosnitskiy Dnіpropetrovsk State Agrarian and Economics University, Dnipro, Ukraine
  • A. А. Sosnitskaya Dnіpropetrovsk State Agrarian and Economics University, Dnipro, Ukraine
Keywords: reference culture, M. avium IEKVM-UAAS, passenger, biological activity, pathogenicity, cultural properties, biological research, immunogenesis, resistance.

Abstract

Mycobacterial diseases of animals and humans are emergent anthropozoonotic infectious pathologies, which bring enormous economic losses to livestock and irreparable sanitary and epidemic damage to the human population throughout the history of civilization. Despite the rapid and effective development of the biological branches of science and the emergence of molecular genetic methods of research, many, even methodologically relatively simple questions of bacteriology of mycobacteria have not been fully studied.One of these aspects is the cultural dissociation of mycobacterial culture at perennial passages on elective nutrient media at optimal temperatures, creating sparing regimens for the growth of microbionts and incorporating the genetic program of saprophyticization. This is a banal situation for any laboratory leading a museum of living cultures. In the bioprospecting industry, in the production of anti-tuberculosis biologics, the cultural variability of reference cultures can lead to the loss of activity of diagnostic drugs. Therefore, it is necessary to monitor the cultural and morphological properties and biological activity of reference crops in the process of repeated re-entry and forecast on the basis of objective information of the biological correspondence of variability variants and its permissible variations from the initial prototype.It has been shown that the M. avium IEKVM-UAAS reference culture has not changed the morphotinic, biochemical and antigenic properties in the course of many years of travel, but the virulence, as the most important component of the biological activity of the strain, has significantly decreased for standard laboratory models and it needs to culture through The organism of a susceptible animal.In a bioassay on chickens and rabbits, the reference of saprophyticization strain exhibited heterogeneous biological activity. On an evolutionarily adapted form of chickens, the culture induced a factor type of the infectious process of low intensity, with a slightly pronounced pathogenic effect, while refreshed re-isolates were obtained. On rabbits - the infectious process was abortive with complete release from the pathogen. 

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Author Biography

A. I. Sosnitskiy, Dnіpropetrovsk State Agrarian and Economics University, Dnipro, Ukraine
д. вет. н, професор

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How to Cite
Sosnitskiy, A., & Sosnitskaya, A. (1). Study of cultural properties and biological activity Mycobacterium avium IEKVM-UAAS. Theoretical and Applied Veterinary Medicine, 5(2), 54-59. Retrieved from https://bulletin-biosafety.com/index.php/journal/article/view/111
Section
Microbiology, Virology and Biotechnology in Animal Industry and Veterinary Medicine