Distribution of larvae of nematode Eustrongylides excisus Jägerskiöld, 1909 (nematoda: dioctophymatidae) in the body of predatory fish
AbstractResults of ichtyopatological examination of predatory fish species (pike-perch – Sander lucioperca (Linnaeus, 1758), perch – Perca fluviatilis (Linnaeus, 1758) and perch – Esox lucius (Linnaeus, 1758) revealed that identified larvae of nematodes (Eustrongylides excisus) mostly were accumulated in fish soft tissues. 346 individuals belonging to three predator species: perch – 155 fishes, pike-perch – 74 fishes, and pike – 117 fishes were examined during 2014 – 2016 years. Fish was randomly sampled during test fishing, caught on fishing rods or purchased from fishermen within the studied area. Fish was sampled along the coastline of the Dnipro-Buh estuary and Dnipro river delta, within the administrative boarders of Mykolayiv region (near the village of Dniprovske in Ochakiv district, Cape Adzhigol) and in the area, which belongs to Kherson region (near the villages Aleksandrovka, Stanislav, Sofiivka, of Belozersky district; nearby villages Rybalche and Heroyske, of Hola Prystan district). Ichthyopathological surveillance of all predator fish: perch, pike-perch and pike was performed. Careful examination of fish skin surface was performed first, than oral cavity and gills were examined separately. Longitudinal dissection was performed on a ventral side of the body from anus to head. All organs were extracted separately, including intestines with its contents. Muscles were dissected in multiple areas and each section was carefully investigated. Brain, spinal cord and eye lens were also examined. 42.7% of Eustorgilidosis larvae were localized in the intercostal muscles, 26.8% of parasites were localized in the ventral part of the abdominal muscles. In the dorsal muscles E. excisus larvae were found less frequently, their total share was 12.3 %. Gonads and hepatopancreas were significantly less affected and accumulated 3.1% and 6.4% of larvae respectively. 2.3% of parasites were found free-floating in the abdominal cavity. The least amount of larvae was found in the walls of the hollow organs – 1.9% of the total number of parasites E. excisus. In the tissues invaded by larvae, signs of inflammatory response, such as hyperemia and edema, were observed. The formation of cysts on the inner layer of the abdominal wall and swelling of the tissues were also detected.
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