Study on the optimal mixotrophic and heterotrophic conditions for lipid accumulation of Chlorella vulgaris

  • O. M. Onyshchenko Dnіpropetrovsk State Agrarian and Economic University, Dnipro
  • A. I. Dvoretsky Dnіpropetrovsk State Agrarian and Economic University, Dnipro
Keywords: microalgae, renewable biomass, husbandry wastes utilization, lipid production


An investigation on productivity and lipid yield of Chlorella vulgaris, a freshwater unicellular alga belonging to the Chlorophyceae class, in a two-stage growing – autotrophic / mixotrophic culturing with manure used as a base for culturing medias and heterotrophic culturing with application of dimethylsulfoxide to induce “lipid trigger” is presented. Combinations of nitrogen starvation, organic substrate and toxicant (dimethylsulfoxide) application are designed to turn the metabolism into an anabolic lipid-accumulating phase. To identify best strategy to induce lipid accumulation for microalgae small and high dosages were evaluated. Regimes of autotrophy and heterotrophy with different light conditions, algal concentration and culturing media composition resulted in high lipid accumulation for Chlorella vulgaris (22,8–61 %), different lipid composition (i.e., polar or nonpolar) accumulated upon different regimes for culture (toxicant concentration) is highlighted.


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How to Cite
Onyshchenko, O., & Dvoretsky, A. (1). Study on the optimal mixotrophic and heterotrophic conditions for lipid accumulation of Chlorella vulgaris. Theoretical and Applied Veterinary Medicine, 5(3), 10-15. Retrieved from