Clinical symptoms and pathological-anatomic changes in the organism of swine for circovirus infection
AbstractThe author based on clinical, pathologic-anatomical and quantitative PCR-analysis determined clinical signs and pathological-anatomical changes at the circovirus infection of pigs. Pathologic-anatomical changes are described depending on the stage of the disease development. The purpose of the study is to establish clinical signs and pathological-anatomical changes at the pig's organism of diseased with circovirus infection. The work was carried out in pig farms in Ukraine, with intensive technology of growing pigs (excommunication from mothers at 28 days old). Studied the peculiarities of the clinical manifestation of circovirus infection of pigs. Corpses of the pigs, who died from circovirus infection (n = 16), underwent a pathologic-anatomical autopsy. PCV-2 was detected in tissues of dead animals by quantitative PCR-analysis. The object of the study is a circovirus infection of pigs. Subject of research: clinical signs and pathologic-anatomical changes in the organism of 8-16 week-old piglets who diseased circovirus infection. Methods of research: clinical (study of the clinical condition of pigs, diseased with circovirus infection), pathological-anatomical change (determination of the main pathological processes in the organism of the diseased of circovirus infection of pigs) and molecular genetic – PCR (detection and identification of PCV-2 in pathological material from the dead pigs). Circovirus infection of pigs in investigated industrial pig-breeding farms with intensive growing technology clinically begins to appear 3-4 weeks after weaning piglets from mothers with subsequent development in 8-16 week-old piglets of the syndrome of multisystem exhaustion and dermato-nephrotic syndrome. Pathological-anatomical changes in the organs of the immune system and in the parenchymal organs of the dead piglets (lymph nodes, spleen, lungs, heart, liver, kidneys) indicate a multisystem nature of the disease and were characterized by circulatory disturbances, dystrophic and proliferative-inflammatory processes characteristic of circovirus infection of pigs. In the stage of initial infection pathological-anatomical changes in organs were characterized by hyperplasia phenomena, and in the stage of breakout infection and the stage of resolution, destructive changes began to prevail with the extinction of proliferative processes, which was combined with sclerotization processes. The use of the clinical and pathologic-anatomical research, along with the molecular-genetic method (quantitative PCR analysis), is necessary for the diagnosis of circovirus infection of pigs, since isolated PCV-2 antigen does not always causes develop syndrome of multisystem depletion
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