Clinical symptoms and pathological-anatomic changes in the organism of swine for circovirus infection

  • V. V. Evert Dnіpropetrovsk State Agrarian and Economic University, Dnipro
Keywords: circovirus infection of pigs, clinical manifestation, pathological changes, diagnosis, PCR-analysis, syndrome of multisystem exhaustion, dermato-nephrotic syndrome

Abstract

The author based on clinical, pathologic-anatomical and quantitative PCR-analysis determined clinical signs and pathological-anatomical changes at the circovirus infection of pigs. Pathologic-anatomical changes are described depending on the stage of the disease development. The purpose of the study is to establish clinical signs and pathological-anatomical changes at the pig's organism of diseased with circovirus infection. The work was carried out in pig farms in Ukraine, with intensive technology of growing pigs (excommunication from mothers at 28 days old). Studied the peculiarities of the clinical manifestation of circovirus infection of pigs. Corpses of the pigs, who died from circovirus infection (n = 16), underwent a pathologic-anatomical autopsy. PCV-2 was detected in tissues of dead animals by quantitative PCR-analysis. The object of the study is a circovirus infection of pigs. Subject of research: clinical signs and pathologic-anatomical changes in the organism of 8-16 week-old piglets who diseased circovirus infection. Methods of research: clinical (study of the clinical condition of pigs, diseased with circovirus infection), pathological-anatomical change (determination of the main pathological processes in the organism of the diseased of circovirus infection of pigs) and molecular genetic – PCR (detection and identification of PCV-2 in pathological material from the dead pigs). Circovirus infection of pigs in investigated industrial pig-breeding farms with intensive growing technology clinically begins to appear 3-4 weeks after weaning piglets from mothers with subsequent development in 8-16 week-old piglets of the syndrome of multisystem exhaustion and dermato-nephrotic syndrome. Pathological-anatomical changes in the organs of the immune system and in the parenchymal organs of the dead piglets (lymph nodes, spleen, lungs, heart, liver, kidneys) indicate a multisystem nature of the disease and were characterized by circulatory disturbances, dystrophic and proliferative-inflammatory processes characteristic of circovirus infection of pigs. In the stage of initial infection pathological-anatomical changes in organs were characterized by hyperplasia phenomena, and in the stage of breakout infection and the stage of resolution, destructive changes began to prevail with the extinction of proliferative processes, which was combined with sclerotization processes. The use of the clinical and pathologic-anatomical research, along with the molecular-genetic method (quantitative PCR analysis), is necessary for the diagnosis of circovirus infection of pigs, since isolated PCV-2 antigen does not always causes develop syndrome of multisystem depletion

Downloads

Download data is not yet available.

References

Allan, G. M., McNeilly, F., Cassidy, J. P., Reilly, G. A. C., Adair, B., Ellis, W. A., & McNulty, M. S. (1995). Pathogenesis of porcine circovirus; experimental infections of colostrum deprived piglets and examination of pig foetal material. Veterinary Microbiology, 44(1), 49–64.

Allan, G. M., McNeilly, F., Kennedy, S., Daft, B., Ellis, J. A., Haines, D. M., … Adair, B. M. (1998). Isolation of Porcine Circovirus-like Viruses from Pigs with a Wasting Disease in the USA and Europe. Journal of Veterinary Diagnostic Investigation, 10(1), 3–10.

Balasch, M., Segalés, J., Rosell, C., Domingo, M., Mankertz, A., Urniza, A., & Plana-Durán, J. (1999). Experimental Inoculation of Conventional Pigs with Tissue Homogenates from Pigs with Post-weaning Multisystemic Wasting Syndrome. Journal of Comparative Pathology, 121(2), 139–148.

Choi, C., Kim, J., Kang, I. J., & Chae, C. (2002). Concurrent outbreak of PMWS and PDNS in a herd of pigs in Korea. Veterinary Record, 151(16), 484–485.

Darwich, L., Segales, J., & Mateu, E. (2004). Pathogenesis of postweaning multisystemic wasting syndrome caused by Porcine circovirus 2 : an immune riddle. Archives of Virology, 149(5), 857–874.

Ellis, J. (2004). Porcine circovirus-2 and concurrent infections in the field. Veterinary Microbiology, 98(2), 159–163.

Gavrilin, P. N. & Evert, V. V. (2015a). Koncepcija mehanizmov povrezhdenija organov immunnoj sistemy u svinej pri cirkovirusnoj infekcii (sindrom mul'tisistemnogo istoshhenija) [Concept of the mechanism of damage the organs immune system in pigs at circovirus infection (multisystemic wasting syndrome)]. Science and Technology Bulletin of SRC for Biosafety and Environmental Control of AIC, 3(2), 12–20 (in Russian).

Gavrylin, P. M. & Evert, V. V. (2015b) Patomorfolohiya limfatychnykh vuzliv svyney na riznykh stadiyakh rozvytku syndromu mul'tysystemnoho vysnazhennya [Pathomorphology lymphnodes of pigs on various stades of development multisystemic wasting syndrome]. Problemy zooinzheneriyi ta veterynarnoyi medytsyny, 31 (2), 289-293 (in Ukranian).

Gavrylin, P., Prokushenkova, O., Nedzvets'kyy, V. & Masyuk D. (2012). Patomorfolohichni kharakterystyky orhaniv i tkanyn svyney pry tsyrkovirus-asotsiyovanykh syndromakh. Naukovyy visnyk NUBiP Ukrayiny, 172 (3), 53-62 (in Ukranian).

Gavrylina, О. H. & Evert, V. V. (2016). Metodychni osoblyvosti zastosuvannya imunohistokhimichnoyi diahnostyky tsyrkovirusnoyi infektsiyi svyney [Methodical features of diagnosis immunochistochemical circovirus infection pigs]. Problemy zooinzheneriyi ta veterynarnoyi medytsyny, 32 (2), 294-301 (in Ukranian).

Helie, P., Drolet, R., Germain, M. C., & Bourgault, A. (1995). Systemic necrozing vasculitis and glomerulonephritis in grower pigs in southwestern Quebec. The Canadian Veterinary Journal, 36, 150 154.

Herilovych, A. P. (2008). Vyvchennya filohenetychnykh osoblyvostey izolyativ tsyrkovirusu svyney, tsyrkulyuyuchykh v riznykh rehionakh svitu ta rozrakhunok praymeriv dlya dyferentsiatsiyi serotypiv zbudnyka. Vet. medytsyna: mizhvid. temat. nauk. zb. — Kh., 89, 89–96 (in Ukranian).

Kartashov, S.N., Kljuchnikov, A.N., Ermakov, A. M. & Lysak, A.S. (2009). Soputstvujushhie infekcii pri sindrome posleotemnogo mul'tisistemnogo istoshhenija svinej. Trudy Kubanskogo gosudarstvennogo agrarnogo universiteta, 1, 45–47 (in Russian).

Kennedy, S., Moffett, D., McNeilly, F., Meehan, B., Ellis, J., Krakowka, S., & Allan, G. (2000). Reproduction of Lesions of Postweaning Multisystemic Wasting Syndrome by Infection of Conventional Pigs with Porcine Circovirus Type 2 Alone or in Combination with Porcine Parvovirus. Journal of Comparative Pathology, 122(1), 9–24.

Krakowka, S., Ellis, J. A., Meehan, B., Kennedy, S., McNeilly, F., & Allan, G. (2000). Viral Wasting Syndrome of Swine: Experimental Reproduction of Postweaning Multisystemic Wasting Syndrome in Gnotobiotic Swine by Coinfection with Porcine Circovirus 2 and Porcine Parvovirus. Veterinary Pathology, 37(3), 254–263.

Malogolovkin, A.S. (2008). Problema cirkovirusnyh infekcij v patologii zhivotnyh i cheloveka. Veterinarija i kormlenie, 2, 30–31 (in Russian).

Orljankin, B., Aliper, T. & Nepoklonov E. (2002). Sovremennye predstavlenija o cirkovirusah svinej. S.–h. biologija ser. Biologija zhivotnyh, 6, 29–37 (in Russian).

Onuki, A., ABE, K., Togashi, K., Kawashima, K., Taneichi, A., & Tsunemitsu, H. (1999). Detection of Porcine Circovirus from Lesions of a Pig with Wasting Disease in Japan. Journal of Veterinary Medical Science, 61(10), 1119–1123.

Opriessnig, T., Meng, X.-J., & Halbur, P. G. (2007). Porcine Circovirus Type 2–Associated Disease: Update on Current Terminology, Clinical Manifestations, Pathogenesis, Diagnosis, and Intervention Strategies. Journal of Veterinary Diagnostic Investigation, 19(6), 591–615.

Satina, T. A. (2003). Cirkovirusnye infekcii svinej. Vladimir: FGU VNIIZZh.

Sato, K., Akachi, S., Asahi, S., Nishida, T., Takahashi, K., Kubo, M., … Yamanaka, S. (2001). Pathological Findings of Pigs Infected with Porcine Circovirus Type 2. Journal of the Japan Veterinary Medical Association, 54(1), 13–17.

Segalés, J., Allan, G. M., & Domingo, M. (2005). Porcine circovirus diseases. Animal Health Research Reviews, 6(02), 119–142.

Stevenson, G. W., Kiupel, M., Mittal, S. K., & Kanitz, C. L. (1999). Ultrastructure of Porcine Circovirus in Persistently Infected PK-15 Cells. Veterinary Pathology, 36(5), 368–378.

Rosell, C., Segales, J., Ramos-Vara, J. A., Folch, J. M., Rodriguez-Arrioja, G. M., Duran, C. O., … Domingo, M. (2000). Identification of porcine circovirus in tissues of pigs with porcine dermatitis and nephropathy syndrome. Veterinary Record, 146(2), 40–43.

Timina, A.M. (2006). Razrabotka metodov laboratornoj diagnostiki cirkovirusnoj infekcii svinej. Extended abstract of candidate's thesis. Vladimir (in Russian).


Abstract views: 336
PDF Downloads: 69
How to Cite
Evert, V. (1). Clinical symptoms and pathological-anatomic changes in the organism of swine for circovirus infection. Theoretical and Applied Veterinary Medicine, 5(4), 5-9. Retrieved from https://bulletin-biosafety.com/index.php/journal/article/view/156