Detection, control of distribution and evaluation of the effectiveness of curative and preventive measures against swine salmonellosis using ELISA

  • K. O. Krutiy Dnіpropetrovsk State Agrarian and Economic University, Dnipro
  • D. M. Masiuk Dnіpropetrovsk State Agrarian and Economic University, Dnipro
  • A. V. Kokarev Dnіpropetrovsk State Agrarian and Economic University, Dnipro
  • S. H. Kolyada Dnіpropetrovsk State Agrarian and Economic University, Dnipro
  • Yu. V. Hustova Dnіpropetrovsk State Agrarian and Economic University, Dnipro
  • N. Yu. Neverkovets Dnіpropetrovsk State Agrarian and Economic University, Dnipro
Keywords: salmonella, swine, seraprevalence, antibody, medical and preventive measures, ELISA

Abstract

Salmonellosis, an infectious disease caused by bacteria of the genus Salmonella and inflicts substantial economic losses pig farms. The aim of the work was to determine the distribution of salmonella among pigs on the farm and to establish the effectiveness of treatment and prevention measures using ELISA. For serological tests by random sampling, serum received from pigs before and after medical and preventive measures at 25, 39, 53, 67, 81, 95, 109, 123, 137, 151 and 165 days of life. From each age group, blood was taken from five animals. The scheme of medical and preventive measures, ELISA and preventive measures used on the farm was developed taking into account the results of the initial serological study. ELISA research was carried out at the Scientific research centre of biosafety and environmental control resources of the agroindustrial complex of the Dnipropetrovsk State Agrarian and Economic University at the BioTek EL×800 (USA) analyzer-photometer using the Swine Salmonella test (“IDEXX” USA). Specific antibodies to the antigen LPS Salmonella sр. serogroup B, C1 and D found in a titer 1:20. According to the instruction, the sample is positive when the value of S/P is greater than or equal to 10%. Variational statistical processing of the results was carried out using a statistical package (StatSoft Inc. USA). To compare the results, Student's criterion was used. Selected parameters listed below in the text, with the following notation: M – arithmetic mean of S/P, m – mean square deviation. The difference is statistically significant at p ˂ 0.05 and lower. The results of the study of blood samples from animals to the treatment-preventative measures indicate circulation in a herd of eukaryotes of the genus Salmonella. It was determined that the investigated farm is seropositive for salmonellosis, the prevalence of which among the pig population is 85%. It has been determined that passive immunity in piglets to Salmonella antigens stay during the first 39 days of life, after which a “serological window” is formed from 53 to 67 days, which promotes the activation of infection in older animals. It was established that the realization of treatment and prophylactic measures for salmonellosis, formed on the basis of the results of serological monitoring, contributes to decrease the prevalence of the disease in the herd in 5,3 times

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Author Biographies

K. O. Krutiy, Dnіpropetrovsk State Agrarian and Economic University, Dnipro
НДЦ біобезпеки та екологічного контролю ресурсів АПК
D. M. Masiuk, Dnіpropetrovsk State Agrarian and Economic University, Dnipro
НДЦ біобезпеки та екологічного контролю ресурсів АПК
A. V. Kokarev, Dnіpropetrovsk State Agrarian and Economic University, Dnipro
НДЦ біобезпеки та екологічного контролю ресурсів АПК
S. H. Kolyada, Dnіpropetrovsk State Agrarian and Economic University, Dnipro
НДЦ біобезпеки та екологічного контролю ресурсів АПК
Yu. V. Hustova, Dnіpropetrovsk State Agrarian and Economic University, Dnipro
НДЦ біобезпеки та екологічного контролю ресурсів АПК
N. Yu. Neverkovets, Dnіpropetrovsk State Agrarian and Economic University, Dnipro
НДЦ біобезпеки та екологічного контролю ресурсів АПК

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Krutiy, K., Masiuk, D., Kokarev, A., Kolyada, S., Hustova, Y., & Neverkovets, N. (1). Detection, control of distribution and evaluation of the effectiveness of curative and preventive measures against swine salmonellosis using ELISA. Theoretical and Applied Veterinary Medicine, 5(4), 25-30. Retrieved from https://bulletin-biosafety.com/index.php/journal/article/view/159