Resistance of the main pathogens of mastitis of cows to modern antimicrobial drugs
AbstractIn the anti-mastitis control approach one of the leading places belongs to its treatment with the use of antibacterial substances. However, their incorrect and uncontrolled use leads to the emergence of antibiotic resistant strains of microorganisms. The purpose of the research is to determine the resistance of the main pathogens of mastitis in cows to modern antimicrobial drugs on dairy farms in the western region of Ukraine in order to implement effective therapeutic and prophylactic anti-mastitis measures. Experimental researches were carried out in the laboratories of Ternopil experimental station of the Institute of veterinary medicine of NAAS (National Academy of Agrarian Sciences of Ukraine), State Agrarian and Engineering University in Podilya and on the farms of Ternopil, Khmelnytsky, Chernivtsi and Lviv regions. The determination of the resistance of microorganisms to antimicrobial drugs was carried out on meat-and-peptone agar (MPA) applying Kirby Bauer Disk Diffusion Method and using standard techniques. The preparation of microbial suspensions was performed according to optical turbidity standards of 1.0 units of McFarland scale using the Densi-LaMeter device (PLIVA-Lachema Diagnostika, Czech Republic). While conducting the research we used non-parametric methods of Wilcoxon and Mann-Whitney tests. It was found that 66.7–83.3% of S. aureus cultures were resistant to 33.3% of preparations and exhibited 100% resistance to 58.3% of preparations. A similar condition was observed in the resistance of S. agalactiae to antibacterial substances which were shedded on dairy farms. The results of studies of resistance of pathogenic microorganisms to anti-mastitis drugs showed that 27.3% of anti-mastitis preparations for cows during lactation showed bactericidal effects on all shedded cultures of S. aureus and S. agalactiae. Up to 22.7% of anti-mastitis drugs showed no effect on pathogens of mastitis. 14.3 to 83.3% of shedded pathogenic microorganisms were sensitive to the remaining drugs. Only one out of three anti-mastitis drugs for dry cows, Bioflok DC (enrofloxacin-based) affected 57.1% of S. aureus microorganisms and 66.7% of S. agalactiae cultures.
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