Morphology of the rabbit sacculus rotundus
AbstractThe sacculus rotundus is a unique anatomical formation, which can be found only in lagomorphs, including the rabbit. It belongs to the rabbit’s intestinal immune formations. It is a hemispherical protrusion of the ileum’s dorsal wall near the area of its transition into the cecum. The data on its morphology in the rabbit are scattered and require refinement. The aim of this study was to reveal morphological and morphometrical properties of the sacculus rotundus and its microscopic structure. The samples were obtained from 12 clinically healthy males 4 months of age of the white Panon breed. The samples were fixed in a 6% neutral buffered formalin solution and embedded in paraffin. Sections (8-10 μm thick) were stained with hematoxylin and eosin, and according to Van Gieson. Microsoft Excel 2010 was used for statistical analysis. The length of sacculus rotundus equaled 4,06 ± 0,1 cm and its maximum height was 2,56 ± 0,08 cm. It had a spongy porous appearance due to the crypts of the mucous membrane. The mucous membrane occupied the largest area of the sacculus rotundus wall – 95,24 ± 0,77%, the muscular layer occupied a significantly smaller area – 3,88 ± 0,41% and the serous membrane – only 0,88 ± 0,36%. The lymphoid tissue occupied most of the mucous membrane area of the sacculus rotundus wall – 71,59 ± 6,92%. Lymphoid tissue was represented by diffuse and nodular forms. The nodular form occupied 96,43 ± 3,33% of lymphoid tissue. Lymph nodules were predominantly secondary and were placed in one row. The height of the lymph nodules was 2530,2 ± 303,39 μm. They were narrow in the area of the dome and their base had the greatest width – 710,68 ± 73,68 μm. The diffuse form occupied only 3,57 ± 3,33% of the lymphoid tissue. Mostly it was located between domes of lymph nodules. The muscular layer consisted of an inner circular layer and a longitudinal outer layer. The serous membrane contained loose connective tissue, which was covered from the outside with a mesentery. In the future it is planned to conduct a study on lymphoid tissue cytological composition of the rabbit sacculus rotundus.
Avtandilov, G. G. (1990). Meditsinskaya morfometrya [Medical morphometry]. Medicine, Moscow (in Russian).
Besoluk, K., Eken, E., & Sur, E. (2012). A morphological and morphometrical study on the sacculus rotundus and ileum of the Angora rabbit. Veterinární Medicína, 51(No. 2), 60–65.
Butler, J. E., & Sinkora, M. (2013). The enigma of the lower gut-associated lymphoid tissue (GALT). Journal of Leukocyte Biology, 94(2), 259–270.
Craigie, E. H. (1948). Bensley's Practical anatomy of the rabbit. The Blakiston Company, Philadelphia.
Goralsky, L. P., Homich, V. T. & Kononsky, O. I. (2016). Osnovi gistologichnoi tekhniku i morfofynctsionalni doslidzhenna y normi ta pru patologii [Basics of gіstologіcal technіk and morfofunctіonal methods of research in norm and pathology]. Polissya, Zhitomir (in Ukrainian).
Haines, R. A., Urbiztondo, R. A., Haynes, R. A. H., Simpson, E., Niewiesk, S. & Lairmore, M. D. (2016). Characterization of New Zealand White Rabbit Gut-Associated Lymphoid Tissues and Use as Viral Oncology Animal Model. ILAR Journal, 57(1), 34–43.
Harcourt-Brown, F. M. (2002). Textbook of Rabbit Medicine. Butterworth Heinemann, United Kingdom. doi:10.1016/B978-075064002-2.50008-4
Mel'nychenko, O.P., Jakymenko, I.L. & Shevchenko, R.L. (2006). Statystychna obrobka eksperymental'nyh danyh [Statistical processing of experimental data]. Bila Cerkva (in Ukrainian).
Nath, S. K., Das, S., Kar, Afrin, K., Dash, A. K. & Akter, Sh. (2016). Topographical and biometrical anatomy of the digestive tract of White New Zealand Rabbit (Oryctolagus cuniculus). Journal of Advanced Veterinary and Animal Research, 3 (2), 145-151.
Ranjan, R., Das, P. & Minj, A. P. (2016). Histomorphological studies on the gut-associated lymphatic tissues (GALT) in rabbit. Indian Journal of Veterinary Anatomy, 28(2), 51-53.
Davies, R. R. & Rees Davies, J. A. (2003). Rabbit gastrointestinal physiology. Veterinary Clinics of North America: Exotic Animal Practice, 6(1), 139–153.
Stojanovs'kyj, V.G., Kolomijec', I. A. & Kamrac'ka, O.I. (2014) Topografichni osoblyvosti imunnyh struktur kyshechnyka kroliv [Topographical features of immune structures of crawls intestine]. Naukovyj visnyk LNUVMBT imeni S.Z. G'zhyc'kogo, 16, 3 (60), 308-313 (in Ukrainian).
Zhedenov, V.N., Byhdan, S. S., Lukianova, V. P., Samborskaia, E. P., Udovyn, H. M. & Yanshyn, K. Y. (1957). Anatomyia krolyka [Rabbit anatomy]. Sovetskaia nauka, Moscow (in Russian).
Abstract views: 87 PDF Downloads: 38