Features of humoral immunity formation under oral and parenteral immunoprophylaxis of porcine epidemic diarrhea

  • D. M. Masiuk Dnipro State Agrarian and Economic University, Dnipro
  • A. V. Kokariev Dnipro State Agrarian and Economic University, Dnipro
  • O. I. Sosnytsky Dnipro State Agrarian and Economic University, Dnipro
  • T. O. Vasilenko Dnipro State Agrarian and Economic University, Dnipro
  • Y. V. Hustova Dnipro State Agrarian and Economic University, Dnipro
Keywords: immunization, IgG, PED, sows, neonatal piglets

Abstract

The research was carried out on the basis of the Scientific Research Center of Biosafety and Environmental Control AIC of the Dnipro State Agrarian and Economic University. The experimental and control groups of second and third farrow sows with 10 heads in each group were formed. Sows of the control group were immunized 21 days before farrowing by individual oral feeding of a virus-containing homogenate tissue of 50 g per animal, which was produced from intestines of suckling piglets who died with clinical manifestations of diarrhea syndrome. Sows of the experimental group were immunized with parenteral injection of an autogenous live vaccine against porcine epidemic diarrhea twice, at 90 and 100 days of fertility at a dose of 2 cm3. Injections were performed intramuscularly in the area behind the ear. Blood samples were taken on 1, 7 and 14 days after farrowing and on 1, 7 and 14 days of life in pigs for the specific IgG analysis. It has been established that parenteral immunization of sows against porcine epidemic diarrhea provides induction of specific IgG and reduces the risk of newborn piglets’ infection in the first days of life. The obtained results show that oral immunization of sows against EDF contributes to seroconversion in 40% of animals of specific IgG in the titre of 1: 200, and for double parenteral immunization, specific IgGs are registered in the diagnostic titer in 70% of sows. Also, an increase in the antibody level in sows of the experimental group was detected by almost 40% in the first 14 days after farrowing against the background of an increase in homogeneity of the S/P index by 14.4% relative to the values in sows after oral immunization. It has been shown that parenteral vaccination of sows contributes to the formation of a specific colostral IgG immunity in 100% of piglets, which is preserved during the first 14 days of life in more than 60% of the animals. Oral immunization of farrowing sows provides the formation of specific IgG immune protection in 60% of animals, which is preserved during the first 14 days of life in only 20% of pigs.

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Published
2018-11-30
How to Cite
Masiuk, D., Kokariev, A., Sosnytsky, O., Vasilenko, T., & Hustova, Y. (2018). Features of humoral immunity formation under oral and parenteral immunoprophylaxis of porcine epidemic diarrhea. Theoretical and Applied Veterinary Medicine, 6(3), 18-22. https://doi.org/10.32819/2018.63004