Erythrocyte system of rat blood during the application of fodder additives of humic nature for combined stress

  • L. M. Diachenko Dnіpro State Agrarian and Economics University, Dnipro
  • L. M. Stepchenko Dnіpro State Agrarian and Economics University, Dnipro
Keywords: erythrocytes, Humilid, Eco-Impulse Animal, alpha-tocopherol, stress


The article is devoted to the research of possibility of preventive influence of antioxidants, feed additives of humic nature on the state of the erythrocytic system under the influence of combined water-immobilization stress. The animals were divided into five groups of 6 animals each: І – the group of intact animals (control); І–V – experimental groups. The animals of all experimental groups were added “Humilid” feed supplement orally (5 mg/kg of body weight per active ingredient), each animal individually with the help of a probe for 18 days, “Eco-Impulse Animal” (2.5 mg/kg of body weight), water and vitamin E (50 mg/kg of body weight). In the later period the animals of ІІ, ІІІ, ІV and V group were conditioned under stress simulation. The blood was analyzed by the number of erythrocytes, erythrocyte content, hematocrit, color index and calculated erythrocytic coefficients. It was established that in animals of group II, after the effect of combined water-immobilization stress, there is a significant decrease in the number of erythrocytes, hemoglobin content and the percentage ratio of formed blood elements is changing. The indices in animals received before the stress period of adding “Humilid”, “Eco Impulse Animal” supplements and vitamin E showed no significant deviations from the control values. However, those rats which before the stress period were added “Eco-Impulse Animal” and vitamin E supplements to the diet, showed a significant increase in the average volume of red blood cells (MCV) and the average content of hemoglobin in erythrocyte (MCH). This fact points to the ability of these substances to influence the processes of blood condensation. The comparison of the influence of antioxidants, “Humilid” and “Eco-Impulse Animal” food additives with vitamin E on the hematological parameters of blood in rats indicates the presence of almost similar effect in the studied substances.


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Diachenko, L., & Stepchenko, L. (2018). Erythrocyte system of rat blood during the application of fodder additives of humic nature for combined stress. Theoretical and Applied Veterinary Medicine, 6(3), 34-38.