Erythrocyte system of rat blood during the application of fodder additives of humic nature for combined stress

  • L. M. Diachenko Dnіpro State Agrarian and Economics University, Dnipro
  • L. M. Stepchenko Dnіpro State Agrarian and Economics University, Dnipro
Keywords: erythrocytes, Humilid, Eco-Impulse Animal, alpha-tocopherol, stress

Abstract

The article is devoted to the research of possibility of preventive influence of antioxidants, feed additives of humic nature on the state of the erythrocytic system under the influence of combined water-immobilization stress. The animals were divided into five groups of 6 animals each: І – the group of intact animals (control); І–V – experimental groups. The animals of all experimental groups were added “Humilid” feed supplement orally (5 mg/kg of body weight per active ingredient), each animal individually with the help of a probe for 18 days, “Eco-Impulse Animal” (2.5 mg/kg of body weight), water and vitamin E (50 mg/kg of body weight). In the later period the animals of ІІ, ІІІ, ІV and V group were conditioned under stress simulation. The blood was analyzed by the number of erythrocytes, erythrocyte content, hematocrit, color index and calculated erythrocytic coefficients. It was established that in animals of group II, after the effect of combined water-immobilization stress, there is a significant decrease in the number of erythrocytes, hemoglobin content and the percentage ratio of formed blood elements is changing. The indices in animals received before the stress period of adding “Humilid”, “Eco Impulse Animal” supplements and vitamin E showed no significant deviations from the control values. However, those rats which before the stress period were added “Eco-Impulse Animal” and vitamin E supplements to the diet, showed a significant increase in the average volume of red blood cells (MCV) and the average content of hemoglobin in erythrocyte (MCH). This fact points to the ability of these substances to influence the processes of blood condensation. The comparison of the influence of antioxidants, “Humilid” and “Eco-Impulse Animal” food additives with vitamin E on the hematological parameters of blood in rats indicates the presence of almost similar effect in the studied substances.

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References

Belay, I. M., Dunaev, V. V., & Tyshkin, V. S. (2001). Investigation of hypolipidemic and antioxidant properties of picamilon and carnitine chloride. Ukrainian Rheumatologic Journal, 1(3), 58–63.

Bordbar, A., Jamshidi, N., & Palsson, B. O. (2011). iAB-RBC-283: A proteomically derived knowledge-base of erythrocyte metabolism that can be used to simulate its physiological and patho-physiological states. BMC Systems Biology, 5(1), 110.

Diachenko, L. M., & Stepchenko, L. M. (2017). Influence of feed additives humic nature on morphological parameters of rat blood. Scientific and Technical Bulletin, 18(2), 71–75.

Gubsky, Yu. I., & Yurshenko, N. M. (2006). Influence of peroxide oxidation on the structure of serum lipoproteids. Odessa Medical Officer Journ, 5, 67–73.

Ivanov, I. I. (2001). Mechanisms of protective action of tocopherols in biological membranes and some related questions. Biomembranes, Riga.

Lang, K. S., Duranton, C., Poehlmann, H., Myssina, S., Bauer, C., Lang, F., Wieder, T., & Huber, S. M. (2003). Cation channels trigger apoptotic death of erythrocytes. Cell Death & Differentiation, 10(2), 249–256.

Lang, K. S., Myssina, S., Brand, V., Sandu, C., Lang, P. A., Berchtold, S., Huber, S. M., Lang, F., & Wieder, T. (2003). Involvement of ceramide in hyperosmotic shock-induced death of erythrocytes. Cell Death & Differentiation, 11(2), 231–243.

Makarova, V. G. (Ed.) (2013). Physiological, biochemical and biometric indices of the norm of experimental animals. "LEMA", St. Petersburg (in Russian).

Manno, S., Takakuwa, Y., & Mohandas, N. (2004). Modulation of Erythrocyte Membrane Mechanical Function by Protein 4.1 Phosphorylation. Journal of Biological Chemistry, 280(9), 7581–7587.

Mastaloudis, A., Morrow, J. D., Hopkins, D. W., Devaraj, S., & Traber, M. G. (2004). Antioxidant supplementation prevents exercise-induced lipid peroxidation, but not inflammation, in ultramarathon runners. Free Radical Biology and Medicine, 36(10), 1329–1341.

Myhaylenko, E. O., Dyomshyna, O. O., & Stepchenko, L. M. (2017). Protein and amino acid metabolism in the muscles of broiler chickens Cobb500 during thearment feed additive "Humilid". Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 19(77), 110–116.

Miller, M. W., Miller, W. M., & Battaglia, L. F. (2003). Biological and environmental factors affecting ultrasound-induced hemolysis in vitro: 3. antioxidant (Trolox®) inclusion. Ultrasound in Medicine & Biology, 29(1), 103–112.

Paronik, V. A., Stecchenko, L. M., Diachenko, L.M., Levyi, A. E., & Shevtsova, A. I. (2015) Influence of corvitine and humidid on the state of the oxidative-antioxidant system of rats on the background of adrenaline introduction. Animal Biology, 17(4), 109–114.

Satoshi, S., Kiyoji, T., Hiroyo, K., & Fumio, N. (1989). Exercise-induced lipid peroxidation and leakage of enzymes before and after vitamin E supplementation. International Journal of Biochemistry, 21(8), 835–838.

Skorik, M. V., & Stepchenko, L. M. (2006). Relationship glutathione metabolism of blood parameters of laying hens on the background of hudrohumate. Veterinary medicine, 86, 292–297 (in Ukrainian).

Stepchenko, L. M., & Skorik, M. V. (2006). Condition of erythrocyte antioxidant laying hens for the actions of humic substances. Technical bulletin Scientific Institute of Animal Biology and State Research Control Institute of Veterinary Preparations and Feed Additives, 7(3–4), 137–143 (in Ukrainian).)

Takagi, K., & Okabe, S. (1968). The effects of drugs on the production and recovery processes of the stress ulcer. The Japanese Journal of Pharmacology, 18(1), 9–18.

Weiner, H. (1996). Use of Animal Models in Peptic Ulcer Disease. Psychosomatic Medicine, 58(6), 524–545.


Abstract views: 191
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Published
2018-11-30
How to Cite
Diachenko, L., & Stepchenko, L. (2018). Erythrocyte system of rat blood during the application of fodder additives of humic nature for combined stress. Theoretical and Applied Veterinary Medicine, 6(3), 34-38. https://doi.org/10.32819/2018.63007