Dynamics of leukocytic indices for parvoviral enteritis at dogs

Keywords: parvoviral infection of dogs, hematologic indices, integral indices, endogenous intoxication, immunoreactivity indexes

Abstract

In this article to be spoken about indicators of action of parvoviridae on an organism of animals – qualitative and quantitative characteristics of blood. These indicators change at many pathological reactions and participate in ensuring nonspecific and specific resistance of an organism. With the help of the analysis of integral leukocyte indices based on the formula of blood reflecting the state of neurohumoral homeostasis and immunological reactivity of the organism, the disturbances were first discovered and established humoral and cellular links of the immune system, micro- and macrophage system, reduction of nonspecific protection of the organism and revealed connections between integral hematological parameters and immunity indexes in dogs with  natural parvoviral infections. The purpose of our research was to determine the possibility of evaluating endogenous intoxication in dogs for parvoviral enteritis on the basis of indicators of index leukocyte count of blood. The work was carried out at the Faculty of Veterinary Medicine of Zhytomyr National Agroecological University, as well as in the veterinary clinics of city of Zhytomyr, city of Berdychiv and city of Kyiv from 2013 to 2016 in breeding and non-breeding dogs. The studies on confirming the diagnosis of viral enteritis were carried out using rapid tests VetExpert CPV- Ag and in the veterinary laboratory using ELISA and PCR. The conducted researches showed that in dogs for parvovirus there is a decrease in the indices of integral hematological parameters, namely the leukocyte index, the ratio of lymphocytes and monocytes, index by Garkava, the index of allergy, the lymphocytic granulocytic index and the index of immunoreactivity (by Shabalov), with subsequent elevations below the physiological limit. In interpreting these data, the inhibition of the immune system, in particular the cellular level, leukocyte deficiency and bone marrow suppression, cerebral hypoxia and cytokine deficiency, should be noted. Integral hematological parameters of peripheral blood have been proved to increase the informative value of the general analysis of blood in dogs with infectious diseases and allow to determine not only the degree of reactivity of the organism, but also to assess the level of endogenous intoxication.

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References

Allison, A. B., Kohler, D. J., Ortega, A., Hoover, E. A., Grove, D. M., Holmes, E. C., & Parrish, C. R. (2014). Host-Specific parvovirus evolution in nature is recapitulated by in vitro adaptation to different carnivore species. PLoS Pathogens, 10(11), e1004475.

Bel’skaya, L., Kosenok, V., & Massard, G. (2016). Endogenous intoxication and saliva lipid peroxidation in patients with lung cancer. Diagnostics, 6(4), 39.

Castro, T. X., Cubel Garcia, R., Gonçalves, L. P., Costa, E. M., Marcello, G. C., Labarthe, N. V., & Mendes-de-Almeida, F. (2013). Clinical, hematological, and biochemical findings in puppies with coronavirus and parvovirus enteritis. The Canadian veterinary journal, 54(9), 885–8.

Derho, M. A., & Samoylova, E. S. (2011) Integralnye indeksy intoksikacii kak kriterij ocenki urovnya endogennoj intoksikacii pri babezioze [Integral indices of intoxication as a criterion for assessing the level of endogenous intoxication with babesiosis]. Journal Scientific Notes Kazan Bauman State Academy of Veterinary Medicine, 207, 177–182 (in Russian).

Duffy, A., Dow, S., Ogilvie, G., Rao, S., & Hackett, T. (2010). Hematologic improvement in dogs with parvovirus infection treated with recombinant canine granulocyte-colony stimulating factor. Journal of Veterinary Pharmacology and Therapeutics, 33 (4), 352–358.

Goddard, A., & Leisewitz, A. L. (2010). Canine Parvovirus. Veterinary Clinics of North America: Small Animal Practice, 40(6), 1041–1053.

Godlevsky, A. I., & Savolyuk, S. I. (2015) Dіagnostika ta monіtoring endotoksikozu u xіrurgіchnix xvorix [Diagnosis and monitoring of endotoxicosis in surgical patients]. Vinnitsa: The New Book, 232 (in Ukrainan).

Godsall, S. A., Clegg, S. R., Stavisky, J. H., Radford, A. D., & Pinchbeck, G. (2010). Epidemiology of canine parvovirus and coronavirus in dogs presented with severe diarrhoea to PDSA PetAid hospitals. Veterinary Record, 167(6), 196–201.

Grin, V. K., Fistal, E. Ya., & Speransky, I. I. (2006) Integralnye gematologicheskie pokazateli lejkocitarnoj formuly kak kriterij ocenki tyazhesti techeniya ozhogovoj bolezni, ee oslozhnenij i effektivnosti provodimogo lecheniya [Integral hematological parameters of leukocyte formula as a criterion for assessing the severity of the course of a burn disease, its complications and the effectiveness of the treatment being performed]. Materials of the Scientific-Practical. Conf. “Sepsis: Problems of Diagnosis, Therapy and Prevention”, March 29-30, Kharkiv, 77–78 (in Russian).

Gutyj, B., Grymak, Y., Drach, M., Bilyk, O., Matsjuk, O., Magrelo, N., Zmiya, M., & Katsaraba, O. (2017). The impact of endogenous intoxication on biochemical indicators of blood of cows with calves. Regulatory Mechanisms in Biosystems, 8(3), 438–443.

Horalskyi, L. P., Radzykhovskyi, M. L., & Dyshkant, O. V. (2018). Intehralni hematolohichni indeksy otsinky stupenia endohennoi intoksykatsii u sobak [Integral hematological indices for assessing the degree of endogenous intoxication in dogs]. Metodychni rekomendatsiyi. Zhytomyr, 28 (in Ukrainan).

Ivashchuk, S. I. (2014). Reaktyvna vidpovid polimorfnoiadernykh neitrofilnykh hranulotsytiv peryferiinoi krovi khvorykh na hostryi pankreatyt zalezhno vid genezu [Reactive response of polymorphonuclear neutrophilic granulocytes of peripheral blood of patients with acute pancreatitis depending on the genesis]. Ukrainian Journal of Surgery, 3–4(26–27), 86–93 (in Ukrainan).

Kilian, E., Suchodolski, J. S., Hartmann, K., Mueller, R. S., Wess, G., & Unterer, S. (2018). Long-term effects of canine parvovirus infection in dogs. PLOS ONE, 13(3), e0192198.

Kholodkovskaya, V. D. (2015). Using integral hematological indices to assess severity of endogenous toxicosis in chronic dermatoses. International Scientific and Practical Conference “World Science”, 3(3), 2, 69–72.

Kochetov, А. G., Lyang, O. V., Masenko, V. P., Zhirov, I. V., Nakonechnikov, S. N., & Tereshchenko, S. N. (2012) Metody statisticheskoj obrabotki medicinskix dannyx: metodicheskie rekomendacii dlya ordinatorov i aspirantov medicinskix uchebnyx zavedenij, nauchnyx rabotnikov [Methods of statistical processing of medical data: Methodical recommendations for interns and post-graduate students of medical educational institutions, scientific workers]. Moscow: RKNPK, 42 (in Russian).

Kumar, M., Nandi, S., & Chidri, S. (2010). Development of a polyclonal antibody-based AC-ELISA and its comparison with PCR for diagnosis of canine parvovirus infection. Virologica Sinica, 25(5), 352–360.

Lobo, A., Vinodkumar, K., Tresamol, P. V., Justin Davis, K., & Priya, P. M. (2017) Hematological changes in dogs with parvovirus enteritis in thrissur district. Imperial Journal of Interdisciplinary Research, 3(6), 1323–1325.

Park, S. A., Park, S.-Y., Song, C.-S., Choi, I.-S., Kim, H. Y., Lee, J.-B., & Lee, N.-H. (2012). Development of a novel vaccine against canine parvovirus infection with a clinical isolate of the type 2b strain. Clinical and Experimental Vaccine Research, 1(1), 70.

Radzykhovsʹkyy, M. L. (2016). Monitorynh enterytiv virusnoyi etiologiyi u sobak [Monitoring enteritis viral etiology in dogs]. Scientific Messenger of National University of Veterinary Medicine and Biotechnologies named after S.Z. Gzhytsky. 18, 1(65), 1, 138–142 (in Ukrainian).

Radzykhovsʹkyy, M. L., Dyshkant, O. V., & Rozumnyuk, A. V. (2016). Hematolohichni ta biokhimichni pokaznyky krovi sobak urazhenykh parvovirusnym enterytom [Hematological and biochemical indices of blood of dogs affected by parvovirus enteritis]. Veterynarna Biotekhnolohiya Byuletenʹ Instytut Veterynarnoyi Medytsyny NAAN, 29, 226–232 (in Ukrainan).

Radzykhovsʹkyy, M. L. (2017). Pokaznyky erytrotsytopoezu u sobak za parvovirusnoho enterytu [Indicators of erythrocytopopse in dogs for parvovirus vulgaris]. Naukovyy Visnyk Veterynarnoyi Medytsyny, 2(136), 97–101 (in Ukrainan).

Sakovich, A. R. (2012). Gematologicheskie lejkocitarnye indeksy pri ostrom gnojnom sinusite [Hematological leukocyte indices in acute purulent sinusitis]. AR Saich UNO “Belarusian State Medical University”, 89–91 (in Russian).

Schaudien, D., Polizopoulou, Z., Koutinas, A., Schwab, S., Porombka, D., Baumgartner, W., & Herden, C. (2010). Leukoencephalopathy associated with parvovirus infection in cretan hound puppies. Journal of Clinical Microbiology, 48(9), 3169–3175.

Showkat, A. S., Sood, N. K, Wani, N., Kuldip, G. & Singh, A. (2013). Haemato-biochemical changes in canine parvoviral infection. Indian Journal of Veterinary Pathology, 37(2) 131–133.

Speransky, I. I., Samoilenko, G. E., & Lobacheva, M. V. (2009). Obshhij analiz krovi ‒ vse li ego vozmozhnosti ischerpany? integralnye indeksy intoksikacii kak kriterii ocenki tyazhesti techeniya endogennoj intoksikacii, ee oslozhnenij i effektivnosti provodimogo lecheniya [General blood test – is all his capabilities exhausted? Integral indices of intoxication as criteria for assessing the severity of the endogenous intoxication course, its complications and the efficacy of the treatment being performed]. Health of Ukraine, 6(19), 51–57 (in Russian).

Sykes, J. E. (2014). Canine parvovirus infections and other viral enteritides. Canine and Feline Infectious Diseases, 141–151.

Tkachenko, E. A., & Derho, M. A. (2014) Lejkocitarnye indeksy pri eksperimentalnoj kadmievoj intoksikacii myshej [Leukocyte indices in experimental cadmium intoxication of mice]. Izvestiya of the Orenburg State Agrarian University, 3(47), 81–83 (in Russian).

Terzungwe, T. M. (2018). Hematological parameters of dogs infected with canine parvovirus enteritis in Sumy Ukraine. World Journal of Innovative Research, 5(3), 1–5.


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Published
2019-03-16
How to Cite
Radsikhovskii, M., Goralskii, L., & Dyshkant, O. (2019). Dynamics of leukocytic indices for parvoviral enteritis at dogs. Theoretical and Applied Veterinary Medicine, 7(1), 3-7. https://doi.org/10.32819/2019.710001