Determination of the chronic toxicity of preparation “Bendamin” on laboratory animals
AbstractIn the process of creating and introducing each new preparation one of the stages is its toxicological studies with the clarification of the parameters of acute and chronic toxicity in white rats in the long term of its intragastric introduction. The aim of the work was to determine the parameters of the chronic toxicity of the cardiological preparation “Bendamin” on laboratory animals in a laboratory experiment. The studies were carried out on white, sexually-mature, young male rats of the Vistar line of 2–3 months old, weighing 170–185 g, which were kept on a standard ration of the Institute Vivarium of the State Scientific-Research Control Institute of Veterinary Preparations and Feed Additives. Four experimental groups of white rats were formed. The first experimental group served as a control. Other experimental groups were prescribed “Bendamin”, respectively, in therapeutic, 5-fold and 10-fold doses. The preparation in the above doses was given at a specific time, daily, during 30 days, peroral, fasting, using a probe for laboratory animals. During the research period to determine the chronic toxicity of “Bendamin”, no probable changes in the behavior of the experimental animals were observed. It should be noted the slight inhibition of the organism state of the being studied group of rats receiving a 10-fold dose of the preparation. In the investigated group of rats, which was injected the preparation in the 10-fold dose, inhibition of protein synthesis and detoxification function of the liver was established. There is also a violation of the functional state of the liver, which indicates an increase in the activity of aminotransferases in their blood. In the study of the relative weight coefficients of the mass of the internal organs, it was found that the introduction of the “Bendamin” preparation in the 10-fold dose led to a significant change in the relative weight of the liver and the spleen. The “Bendamin” preparation in the therapeutic and 5-fold doses contributed to a slight increase in the weighting coefficient. Lung weight loss occurred at introduction of the preparation in the 5- and 10-fold doses. According to State Standard 12.1.007-76, “Bendamin”, for intragastric introduction, according to the degree of hazard belongs to the fourth-class toxicity – low toxicity substances (DL50 more than 50,000 mg/kg body weight).
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