Metabolic profile of blood of pigs infected with рorcine epidemic diarrhea
AbstractPorcine epidemic diarrhea (PED) is an emergent, highly contagious viral disease of pigs of different ages with the development of lethal diarrhea syndrome in non-immune piglets, the causative agent of which is virus Porcine epidemic diarrhea. The main symptom of PED is watery diarrhea with is observed in piglets of 1 week of age are manifested by depressed behavior, lack of appetite, and liquid feces without any signs of blood. The aim of this work was to find out the metabolic profile of the blood of piglets infected with the virus of epidemic diarrhea of pigs. For the study were formed 3 groups of piglets at the age of 16–18 days of life with characteristic clinical signs, selected in each of the examined farms. The first group consisted of 44 animals, the second group – 32, and 6 clinically healthy animals were isolated in a separate group – control. Blood for the study was taken from piglets for 3–4 days after the appearance of clinical signs of PED. Hematological parameters were determined on an automatic hematological analyzer. The ratio of various forms of leukocytes was determined by counting them in stained blood smears. Biochemical studies were carried out by conventional methods using an automatic biochemical analyzer. As a result of the studies, it was found that in case of a disease of epidemic diarrhea in pigs, changes in blood hematological parameters occur, characterized by an increase in the number of red blood cells and white blood, hematocrit and hemoglobin levels with a simultaneous increase in RЕS, which indicates the development of hypovolemic polycythemia on the background of dehydration of sick animals. An increase in the activity of aminotransferases, amylase, alkaline phosphatase and urea level was established, against a background of a decrease in the albumin fraction of the protein, glucose, cholesterol and total lipoproteins, which is due to depletion and intoxication of the body of patients with epidemic diarrhea of pigs. Thus, the results of studies of the metabolic parameters of blood of piglets with clinical signs of PED indicate dehydration, depletion and intoxication of the organism of sick pigs, occurs against the background of damage and destruction of cell small intestine by coronavirus.
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