Metabolic profile of blood of pigs infected with рorcine epidemic diarrhea

Keywords: РED virus; piglets; morphological and biologic blood counts


Porcine epidemic diarrhea (PED) is an emergent, highly contagious viral disease of pigs of different ages with the development of lethal diarrhea syndrome in non-immune piglets, the causative agent of which is virus Porcine epidemic diarrhea. The main symptom of PED is watery diarrhea with is observed in piglets of 1 week of age are manifested by depressed behavior, lack of appetite, and liquid feces without any signs of blood. The aim of this work was to find out the metabolic profile of the blood of piglets infected with the virus of epidemic diarrhea of pigs. For the study were formed 3 groups of piglets at the age of 16–18 days of life with characteristic clinical signs, selected in each of the examined farms. The first group consisted of 44 animals, the second group – 32, and 6 clinically healthy animals were isolated in a separate group – control. Blood for the study was taken from piglets for 3–4 days after the appearance of clinical signs of PED. Hematological parameters were determined on an automatic hematological analyzer. The ratio of various forms of leukocytes was determined by counting them in stained blood smears. Biochemical studies were carried out by conventional methods using an automatic biochemical analyzer. As a result of the studies, it was found that in case of a disease of epidemic diarrhea in pigs, changes in blood hematological parameters occur, characterized by an increase in the number of red blood cells and white blood, hematocrit and hemoglobin levels with a simultaneous increase in RЕS, which indicates the development of hypovolemic polycythemia on the background of dehydration of sick animals. An increase in the activity of aminotransferases, amylase, alkaline phosphatase and urea level was established, against a background of a decrease in the albumin fraction of the protein, glucose, cholesterol and total lipoproteins, which is due to depletion and intoxication of the body of patients with epidemic diarrhea of pigs. Thus, the results of studies of the metabolic parameters of blood of piglets with clinical signs of PED indicate dehydration, depletion and intoxication of the organism of sick pigs, occurs against the background of damage and destruction of cell small intestine by coronavirus.


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Chen, Q., Gauger, P. C., Stafne, M. R., Thomas, J. T., Madson, D. M., Huang, H., Zheng, Y., Li, G., & Zhang, J. (2016). Pathogenesis comparison between the United States porcine epidemic diarrhoea virus prototype and S-INDEL-variant strains in conventional neonatal piglets. Journal of General Virology, 97, 1107–1121.

Crawford, K., Lager, K., Miller, L., Opriessnig, T., Gerber, P., & Hesse, R. (2015). Evaluation of porcine epidemic diarrhea virus transmission and the immune response in growing pigs. Veterinary Research, 46–49.

Hu, H., Jung, K., Vlasova, A. N., Chepngeno, J., Lu, Z., Wang, Q., & Saif, L. J. (2015). Isolation and characterization of porcine deltacoronavirus from pigs with diarrhea in the United States. Journal of Clinical Microbiology, 53, 1537–1548.

Jung, K., Eyerly, B., Annamalai, T., Lu, Z., & Saif, L. J. (2015). Structural alteration of tight and adherens junctions in villous and crypt epithelium of the small and large intestine of conventional nursing piglets infected with porcine epidemic diarrhea virus. Veterinary Microbiology, 177, 373–378.

Jung, K., Wang, Q., Scheuer, K. A., Lu, Z., Zhang, Y., & Saif, L.J. (2014). Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs. Emerging Infectious Diseases Journal, 20, 662–665.

Kocherhans, R., Bridgen, A., Ackermann, M., & Tobler, K. (2001). Completion of the porcine epidemic diarrhoea coronavirus (ЕДСV) genome sequence. Virus Genes, 23, 137–144.

Lee, C. (2015). Porcine epidemic diarrhea virus: An emerging and re-emerging epizootic swine virus. Virology Journal, 12(193), 1–16.

Lin, C. M., Gao, X., Oka, T., Vlasova, A. N., Esseili, M. A., Wang, Q., & Saif, L. J. (2015). Antigenic relationships among porcine epidemic diarrhea virus and transmissible gastroenteritis virus strains. Journal of Virology, 89, 3332–3342.

Lowe, J., Gauger, P., Harmon, K., Zhang, J., Connor, J., Yeske, P., Loula, T., Levis, I., Dufresne, L., & Main, R. (2014). Role of transportation in spread of porcine epidemic diarrhea virus infection, United States. Emerging Infectious Diseases Journal, 20 (5), 872–874.

Masiuk, D. N., Nedzvetsky, V. S., Sosnitskiy, A. I., Kokarev, A. V., & Koliada, S. G. (2018). The characteristics, emergent properties and manner of spread in Ukraine of the Porcine Epidemic Diarrhea Virus. Regulatory Mechanisms in Biosystems, 9(3), 401–408.

Miller, L. C., Crawford, K. K., Lager, K. M., Kellner, S. G., & Brockmeier, S. L. (2016). Evaluation of two real-time polymerase chain reaction assays for Porcine epidemic diarrhea virus (ЕДСV) to assess ЕДСV transmission in growing pigs. Journal of Veterinary Diagnostic Investigation, 28, 20–29.

Prapassorn, B., Alongkot, B., Urai, P., & Tanatpon, P. (2018). The comparison of villous damage at different ages of piglets infected with porcine epidemic diarrhea virus. Chiang Mai Veterinary Journal, 16(1), 37–46.

Pillatzki, A., Gauger, P., Madson, D., Burrough, E., Zhang, J., Chen, Q., Magstadt, D., Arruda, P., Stevenson, G., & Yoon, K.J. (2015). Experimental inoculation of neonatal piglets with feed naturally contaminated with porcine epidemic diarrhea virus (ЕДСV). Journal of Swine Health & Production, 23 (6), 317–320.

Stadler, J., Zoels, S., Fux, R., Hanke, D., Pohlmann, A., Blome, S., Weissenböck, H., Weissenbacher-Lang, C., Ritzmann, M., & Ladinig, A. (2015). Emergence of porcine epidemic diarrhea virus in southern Germany. BMC Veterinary Research, 11, 142.

Stevenson, G. W., Hoang, H., Schwartz, K. J., Burrough, E. R., Sun, D., Madson, D., Cooper, V. L., Pillatzki, A., Gauger, P., Schmitt, B. J., Koster, L. G., Killian, M. L., & Yoon, K. J. (2013). Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences. Journal of Veterinary Diagnostic Investigation, 25, 649–654.

Thomas, J. T., Chen, Q., Gauger, P. C., Gimenez-Lirola, L. G., Sinha, A., Harmon, K. M., Madson, D. M., Burrough, E. R., Magstadt, D. R., Salzbrenner, H. M., Welch, M. W., Yoon, K. J., Zimmerman, J. J., & Zhang, J. (2015). Effect of Porcine epidemic diarrhea virus infectious doses on infection outcomes in naive conventional neonatal and weaned pigs. PLoS One 10:e0139266.

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Masiuk, D. M., Nedzvetsky, V. S., Kokariev, A. V., & VasуlenkoT. О. (2019). Metabolic profile of blood of pigs infected with рorcine epidemic diarrhea. Theoretical and Applied Veterinary Medicine, 7(3), 167-171.