The protein ratio at different poles of the enterocytes’ cell membrane of the jejunum during the fetal stage in cattle

Keywords: polypeptides; jejunum; enterocytes; apical membrane; basolateral membrane.

Abstract

Polarity plays a decisive role in ensuring the functional properties of intestinal enterocytes, the apical membrane is responsible for the hydrolysis and transport of substances, while basolateral membrane is responsible for the entry of substances into the bloodstream. For the research were used 80 two to nine months old cattle fetuses. To obtain apical and basolateral membranes from a suspension of isolated enterocytes from the cattle’s jejunum, the basic method of differential centrifugation in the author’s modification was used. New data on the concentration dynamics of individual protein fractions on the enterocytes apical and basolateral membranes from the jejunum of cattle fetuses are presented. It is established that during the late fetal stage there are dynamic changes in the polypeptide composition of the apical and basolateral membranes of enterocytes, which are characterized not only by changes in their ratio but also by redistribution between the poles of these cells. In the apical and basolateral membranes, respectively, 31 and 27 protein fractions were detected with molecular weights from 9.6 kDa to 300 kDa. At this moment, there is a decrease in the content of low molecular weight protein fractions and an increase in the proportion of high molecular weight ones. The expression and content of different polypeptide fractions on the polar domains of the jejunal enterocytes of 9-month-old cattle fetuses are characterized by a predominance of low molecular weight proteins (from 21 kDa to 33 kDa) in the apical membrane, while in the basolateral – proteins of higher molecular weight are dominant (from 35 kDa to 300 kDa). Thus, the apical membrane has a higher content of proteins with a molecular weight of 17 kDa – 8.48 times more than in basolateral (P ≤ 0.001), 21 kDa – 1.23 times more (P  ≤ 0.001), 22.5 kDa – 6.39 times (P ≤ 0.001), 24 kDa – 1.29 times (P ≤ 0.001), 26 kDa – 1.56 times (P ≤ 0.001), 29 kDa – 1.27 times (P ≤ 0.01), 31 kDa – 1.54 times (P  ≤ 0.001), 33 kDa – 1.61 times (P ≤ 0.001) and 46 kDa – 1.56 times (P ≤ 0.01). In contrast, in the enterocytes basolateral membrane there is a higher content of polypeptide fractions compared to that in the apical membrane, with a molecular weight of 15.5 kDa – 3.64 times more (P ≤ 0.001), 35 kDa – 1.63 times (P ≤ 0.01), 39 kDa – 1.88 times (P ≤ 0.001), 43 kDa – 1.87 times (P ≤ 0.001), 52 kDa – 1.26 times (P ≤ 0.001), 63 kDa – 1.11 times (P ≤ 0.05), 66 kDa – 1.29 times (P ≤ 0.001), 87 kDa –  3.16 times (P ≤ 0.001), 100 kDa –  3.03 times (P ≤ 0.001), 155 kDa – 1.15 times (P ≤ 0.05), 170–185 kDa – 1.50 times (P ≤ 0.001) and 300 kDa – 1.59 times (P ≤0.001).

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References

Abdullahi, W., Brzica, H., Hirsch, N. A., Reilly, B. G., & Ronaldson, P. T. (2018). Functional expression of organic anion transporting polypeptide 1a4 is regulated by transforming growth factor-β/activin receptor-like kinase 1 signaling at the blood-brain barrier. Molecular Pharmacology, 94(6), 1321–1333.

Almássy, J., Diszházi, G., Skaliczki, M., Márton, I., Magyar, Z. É., Nánási, P. P., & Yule, D. I. (2019). Expression of BK channels and Na+-K+ pumps in the apical membrane of lacrimal acinar cells suggests a new molecular mechanism for primary tear-secretion. The Ocular Surface, 17(2), 272–277.

Fölsch, H. (2008). Regulation of membrane trafficking in polarized epithelial cells. Current Opinion in Cell Biology, 20(2), 208–213.

Gajda, A. M., & Storch, J. (2015). Enterocyte fatty acid-binding proteins (FABPs): different functions of liver and intestinal FABPs in the intestine. Prostaglandins, Leukotrienes and Essential Fatty Acids, 93, 9–16.

Gessner, J. E., Heiken, H., Tamm, A., & Schmidt, R. E. (1998). The IgG Fc receptor family. Annals of Hematology, 76(6), 231–248.

Grandl, F., Schwarm, A., Ortmann, S., Furger, M., Kreuzer, M., & Clauss, M. (2017). Kinetics of solutes and particles of different size in the digestive tract of cattle of 0.5-10 years of age, and relationships with methane production. Journal of Animal Physiology and Animal Nutrition, 102(3), 639–651.

Holopura, S. I., Maryniuk, M. O., & Tsvilikhovskyi, M. I. (2017). Ekspresiia imunoretseptornykh proteiniv u plazmolemi enterotsytiv novonarodzhenykh teliat u period formuvannia kolostralnoho imunitetu [Expression of imunoreceptor proteins in plasmolemma of enterocytes in formation of colostric immunity in newborn calves]. The Animal Biology, 19(2), 16–22 (in Ukrainian).

Masiuk, D. M. (2004a). Methodological features of obtaining and structural characterization of isolated enterocytes of the small intestine epithelium of cattle. Bulletin of the National Agrarian University, 75, 148–152 (in Ukrainian).

Masiuk, D. M. (2004b). Features of fractionation of plasma membranes isolated from small intestine enterocytes of cattle. Bulletin of the National Agrarian University, 78, 125–129 (in Ukrainian).

Masiuk, D. M. (2019). Structural proteins of plasmolemma of the jejunum absorbing enterocytes of cattle fetus in early fetal period. Ukrainian Journal of Veterinary and Agricultural Sciences, 2(3), 32–38.

Melnychuk, D. O., Liubetska, T. V., Tsvilikhovskyi, M. I., Hryshchenko, V. A., & Yakymchuk, O. M. (2002). Postnatalni biokhimichni protsesy v orhanizmi novonarodzhenykh teliat [Postnatal biochemical process in the body of newborn calves]. Ukrainian Biochemical Journal, 74(4B/2), 98 (in Ukrainian).

Nejsum, L. N., & Nelson, W. J. (2007). A molecular mechanism directly linking E-cadherin adhesion to initiation of epithelial cell surface polarity. Journal of Cell Biology, 178(2), 323–335.

Rodriguez-Boulan, E., & Powell, S. K. (1992). Polarity of epithelial and neuronal cells. Annual Review of Cell Biology, 8(1), 395–427.

St Johnston, D., & Sanson, B. (2011). Epithelial polarity and morphogenesis. Current Opinion in Cell Biology, 23(5), 540–546.

Tarabova, L., Makova, Z., Piesova, E., Szaboova, R., & Faixova, Z. (2016). Intestinal mucus layer and mucins (A review). Folia Veterinaria, 60(1), 21–25.

Tilney, L. G., Hatano, S., Ishikawa, H., & Mooseker, M. S. (1973). The polymerization of actin: its role in the generation of the acrosomal process of certain echinoderm sperm. The Journal of Cell Biology, 59(1), 109–126.

Tomchuk, V. A., Usatiuk, P. V., Tsvilikhovskyi, M. I., & Melnychuk, D. O. (1994) Otrymannia izolovanykh klityn epiteliiu tonkoho kyshechnyka velykoi rohatoi khudoby [Obtaining isolated cells of the epithelium of the small intestine of cows]. Physiological Journal, 40(5/6), 45–51 (in Ukrainian).

Tsvilikhovskyi, M. I., Bereza, V. I., Nemova, T. V., & Yakymchuk, O. M. (2014). Limitni faktory i patolohiia tvaryn antenatalnoho ta postnatalnoho rozvytku [Limiting factors and pathology of animals of antenatal and postnatal development]. Bulletin of the Poltava State Agrarian Academy, 3, 92–94 (in Ukrainian).

Tsvilikhovskyi, N. I. (1989). Vydelenie apikal’noj i bazal’noj membran jenterocita tonkoj kishki korov i strukturno-funkcional’nye izmenenija v nih pri patologii [Isolation of the apical and basement membranes of enterocyte of the small intestine of cows and structural and functional changes in them during pathology]. Physiological Journal, 35(5), 121 (in Russian).

Vlizlo, V. V., Fedoruk, R. S., & Ratych, I. B. (2012). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni [Laboratory research methods in biology, animal husbandry and veterinary medicine]. Lviv, Spolom (in Ukrainian).


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Published
2020-03-20
How to Cite
Masiuk, D. M. (2020). The protein ratio at different poles of the enterocytes’ cell membrane of the jejunum during the fetal stage in cattle. Theoretical and Applied Veterinary Medicine, 8(1), 62-68. https://doi.org/10.32819/2020.81010