The effect of a new feed additive of humic nature on metabolic processes in laboratory rats depending on the dose

Keywords: «Animal Forte»; laboratory rats; morphofunctional blood parameters; biochemical blood parameters.

Abstract

The effectiveness of the new humic nature feed additive – «Animal Forte», has been established, depending on its amount on the metabolism of laboratory rats. We used sexually mature male white rats of 2 months age, from which five experimental groups were formed and one intact, with eight animals each. The experiment lasted four weeks, during which the animals of the experimental groups received daily in addition to the main feed 5, 10, 20, 30 and 50 mg of feed additive with its active substance per 1 kg of animal’s weight. Morpho-functional indicators (hemoglobin content, hematocrit, red blood cells, platelets, and leukocytes) were determined in EDTA stabilized blood. To define the effectiveness of a new humic nature food supplement, the content of total protein, albumin and globulins, uric acid, urea, creatinine, glucose, and the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT) were measured in blood serum. Red blood cell coefficients were determined by a calculation method: mean erythrocyte volume (MCV); mean erythrocyte hemoglobin content (MCH); mean hemoglobin concentration in erythrocyte (MCHC). A feed supplement at a dose of 10 mg/kg had a greater effect on hematopoietic function, especially on the erythropoiesis processes, namely, the hemoglobin content, the number of RBC, the RBC index – MCV, MCH were increased. Due to this, respiration and oxidation-reduction processes in animal tissue were improved. Usage of a biologically active humic nature substance at a dose of 5 mg/kg of animals weight to a greater extent compared with other options improved the function of liver hepatocytes, increasing its protein-synthesizing property, due to this effect, the total protein and albumin content in the serum of experimental animals were increased. Under the influence of the feed additive on metabolic processes, including the membrane’s permeability, in the blood of rats of experimental groups, the level of glucose was decreasing. The positive effect of Animal Forte on the kidneys filtration and excretory properties was also noted, due to which the content of urea and creatinine in the blood of rats was decreased. It has been established that already with a minimal dose of 5 mg/kg of body weight, the feed additive had an effect on animals’ metabolic processes.

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References

Buchko, O. M. (2015). Vpliv dobavki gumіnovoї prirodi na pokazniki bіlkovogo ta energetichnogo obmіnu v svinej [Influence of humic nature additives on protein and energy metabolism in pigs]. Bulletin of Agricultural Science, 5, 31–35.
Chorna, V. І., Stepchenko, L. M., & Lyanna, O. L. (2010). Osoblyvosti vplyvu biolohichno aktyvnykh rechovyn iz torfu na proteoliz mozku shchuriv za umov modelnoho eksperymentu. Proceedings of the Intrnational Conference «Guminovye veshhestva i fitogormony v sel’skom hozjajstve». Dnipro, 174–175 (in Ukrainian).
Dyachenko, L. M., & Stepchenko, L. M. (2018). Eritrocitarna sistema krovі shchurіv na tlі zastosuvannya kormovih dobavok gumіnovoї prirodi za kombіnovanogo stresu. Theoretical and Applied Veterinary Medicine, 6(3), 34–38.
Dyomshina, O. O., Ushakova, G. O., & Stepchenko, L. M. (2017). The effect of biologically active feed additives of humilid substances on the antioxidant system in liver mitochondria of gerbils. Regulatory Mechanisms in Biosystems, 8(2), 185–190.
Dubishchev, A. V., & Menshih, L. E. (2010). Korrekciya ekologicheskogo neblagopoluchiya. Produkty Pitaniya, 2023–2026 (in Russian).
Garashchuk, M. І. (2012). Vikoristannya gumatu natrіyu z metoyu korekcії obmіnu rechovin u molodnyaka svinej [The use of sodium humate to correct metabolism in young pigs]. Science and Technology Bulletin of SRC for Biosafety and Environmental Control of Agro-Industrial Complex, 1(1), 63–65 (in Ukrainian).
Garashchuk, M. І. (2010). Vikoristannya gumatu natrіyu dlya lіkuvannya gastroenterіtu u porosyat. News of Dnipropetrovsk State Agrarian University, 2, 110–111 (in Ukrainian).
Gordіenko, Yu. A., Baklanova, Ya., Kovalenko, M. V., Stepchenko, L. M., Shevcova, A. І., & Ushakova, G. O. (2012). Zmіni fіzіologіchnih ta bіohіmіchnih pokaznikіv u shchurіv z doksorubіcin-іndukovanoyu kardіopatієyu na tlі zastosuvannya preparatіv z antioksidantnoyu dієyu. The Animal Biology, 14(1-2), 74–79 (in Ukrainian).
Gryban, V., & Pechenyy, E. (2016). Hematological and biochemical blood indices of cows of Ukrainian meat breed under the influence of humilid. Theoretical and Applied Veterinary Medicine, 4(4), 18–21.
Griban, V. G. (2010). Vykorystannia preparativ huminovoi pryrody dlia stymuliatsii rezystentnosti i produktyvnosti tvaryn [The use of drugs of humic nature to stimulate resistance and productivity of animals]. Proceedings of the Intrnational Conference «Guminovye veshhestva i fitogormony v sel’skom hozjajstve». Dnipro, 171–173 (in Ukrainian).
Griban, V. G., & Masyuk, D. M. (1998). Efektivnіst zastosuvannya gіdrogumatu dlya korekcії obmіnu rechovin u glibokotіlnih korіv і profіlaktiki pіslyarodovih zahvoryuvan. News of Dnipropetrovsk State Agrarian University, 1-2, 83 (in Ukrainian).
Gunchak, A. V., Stepchenko, L. M., Ratich, І. B., & Stefanіshin, O. M. (2017). Efektyvnist vykorystannia spoluk huminovoi pryrody v ratsionakh perepilok [The effectiveness of the use of humic compounds in the diets of quails]. News of Dnipropetrovsk State Agrarian University, 2(44), 53–57 (in Ukrainian).
Kushch, M. M., Fesenko, І. A., Kushch, L. L., & Stepchenko, L. M. (2017). Mіkroskopіchnі pokazniki dvanadcyatipaloї kishki gusej 8-mіsyachnogo vіku za vikoristannya gumіlіdu [Microscopic parameters of the duodenum of 8-month-old geese using humilide]. Problemi Zooіnzhenerії ta Veterinarnoї Medicini, 35(2), 188–192 (in Ukrainian).
Komisarov, I. D. (2008). Biologicheskaya aktivnost guminovyh preparatov. Dosyagnennya ta perspektivi zastosuvannya gumіnovih rechovin u sіlskomu gospodarstvі. Dnіpro, 75–78.
Mihajlenko, E. O., Domshina, O. O., Stepchenko, L. M., & Ushakova, G. O. (2016). Antioksidantna sistema pechіnki brojlerіv krosu Kobb-500 v umovah vipoyuvannya prirodno aktivnoyu dobavkoyu na osnovі gumіnovih rechovin. News of Dnipropetrovsk State Agrarian University, 4(12), 120–125.
Paronіk, V. A., Stepchenko, L. M., Dyachenko, L. M., Levih, A. E., & Shevcova, A. І. (2015). Vpliv korvіtinu ta gumіlіdu na stan oksidantno – antioksidantnoї sistemi shchurіv na fonі vvedennya adrenalіnu. The Animal Biology, 17(4), 109–114 (in Ukrainian).
Poluyanova, I. E. (2017). Biologicheskaya aktivnost’ guminovyh veshchestv poluchaemyh iz torfa i vozmozhnosti ih ispol’zovaniya v lechebnoj praktike. Mezhdunarodnye Obzory: Klinicheskaya Praktika i Zdorov’e, 4, 114–122 (in Belarus).
Stepchenko, L., Yefimov, V., Garashchuk, M., Rakytyanskyy, V. (2012). Effective use of peat products in pig industry. Abstracts of the 14-th International Peat Congress. Stockholm, 122.
Stepchenko, L. M. (2010). Regulyatornі mekhanіzmi dії bіologіchno aktivnih rechovin gumіnovoї prirodi na organіzm produktivnoї pticі. Fiziologichnyi Zhurnal, 56(2), 306.
Stepchenko, L. M. (2010). Znachenia katepsinu B ta jogo inhibitoriv v reguljaciji obminnyh procesiv u kurchat-brojleriv za diji rechovyn guminovoji prypody [The value of cathepsin B and its inhibitors in the regulation of metabolism in broiler chickens for the actions of humic substances]. The Animal Biology, 12(2), 180–188 (in Ukrainian).
Stepchenko, L. M., & Skorik, M. V. (2006). Condition of erythrocyte antioxidant laying hens for the actions of humic substances. Technical Bulletin Scientific Institute of Animal Biology and State Research Control Institute of Veterinary Preparations and Feed Additives, 7(3–4), 137–143 (in Ukrainian).
Visser, S. A. (1972). Physiological action of humic acids on living cells. The Proc.4 lnt. Peat Congr. Finland:Ctaniemy, 186–192.
Yefimov, V., & Rakytianskyi, V. (2015). The parameters of cellular immunity of piglets during rearing period under the influence of sodium humate, succinic acids and trace elements. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 17(3), 32–37 (in Ukrainian).

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Published
2020-06-12
How to Cite
StepchenkoL. М., Harashchuk, M. I., Spitsina, T. L., Platonova, T. S., & Smirnov, D. O. (2020). The effect of a new feed additive of humic nature on metabolic processes in laboratory rats depending on the dose. Theoretical and Applied Veterinary Medicine, 8(2), 158-162. https://doi.org/10.32819/2020.82022