Clinical-biochemical status of hens due to changes of battery cages height location

Keywords: laying hens; chronic stress; battery cages; glucose; creatinine; enzyme activity

Abstract

In the conditions of industrial poultry farming, the body of hens is constantly exposed to numerous technological stressors, the least studied of which are long-term ones, that can provoke chronic stress. One such technological stressor is an increase in the level of cage equipment, which is used by producers to obtain a larger amount of products from 1 m2 of poultry area. The aim of this work was to study the physiological state of the hen’s body based on the clinical biochemical analysis of their blood serum, depending on the battery cages height. In the conditions of a modern complex for the production of edible eggs in a poultry house with an area of 2 915 m2, 4 groups of hens were formed, each of them was kept on a separate floor-analogue in terms of area and cage equipment. Each floor was equipped with «Big Dutchman» 3-tier battery cages. Battery cages of each floor were separated from each other by a lattice floor so that cages of 1–3 tiers entered up to the 1st floor, 4–6 tiers – up to the 2nd, 7–9 tiers – up to the 3rd, and 10–12 tiers – up to 4th floor of cage equipment. Biochemical parameters and activity of hens blood serum enzymes were determined on a BioChem FC-360 biochemical analyzer. For this, 30 blood samples with a volume of 1.0–1.5 ml were taken from axillary veins of laying hens in each group at the age of 18 weeks (at the beginning of the study) and at 52 weeks. It was found that increasing the layering of cage equipment does not have a negative impact on the hen’s body. So, for keeping hens in cages of multi-tier battery cages located on floors 2–4 (4–12 tiers), the biochemical status and activity of their blood serum enzymes were within the physiological norm. Whereas, for hens kept in battery cages of the a multi-tiered system on the 1st floor there was an increase in glucose levels of 60.5–71.0%, creatinine – by 9.7–12.3%, phosphorus – 82.6–100.0%, was observed a decrease in calcium to phosphorus ratio 46.7–50.0%, which was confirmed by an increase in the activity of alkaline phosphatase by 22.3–27.0%, as well as an increase in the activity of aspartate aminotransferase by 3.2–13.8%, lactate dehydrogenase - by 48.5–65.1% and gamma-glutamyl transferase – by 16.4–20.6%. It has been proven that the main consequences of chronic stress caused by keeping hens in the lower floor cells of the multi-tiered battery cage are reflected in the biochemical parameters of their serum, namely increased glucose, creatinine, enzyme activity and violation of calcium to phosphorus ratio.

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References

Al-Hussary, A. J. N., & Kudair, M. I. (2010). Effect of vaccination on some biochemical parameters in broiler chickens. Iraqi Journal of Veterinary Sciences, 24(2), 59–64.

Bueno, J. P. R., Nascimento, M. R. B. de M., Martins, J. M. da S., Marchini, C. F. P., Gotardo, L. R. M., Sousa, G. M. R. de, Mundim, A. V., Guimarães, E. C., & Rinaldi, F. P. (2017). Effect of age and cyclical heat stress on the serum biochemical profile of broiler chickens. Semina: Ciências Agrárias, 38(3), 1383.

Downing, J. (2012). Оn-invasive assessment of stress in commercial housing systems. North Sydney, Australian Egg Corporation Limited, 69.

Ericsson, M., Henriksen, R., Bélteky, J., Sundman, A.-S., Shionoya, K., & Jensen, P. (2016). Long-term and transgenerational effects of stress experienced during different life phases in chickens (Gallus gallus). Plos One, 11(4), e0153879.

Goel, A. (2021). Heat stress management in poultry. Journal of Animal Physiology and Animal Nutrition, 105(6), 1136–1145.

González, F. H. D., & Silva, S. C. (2006). Introdução à bioquímica clínica veterinária. 2. ed. Porto Alegre: Universidade Federal do Rio Grande do Sul, 364.

Guo, Y., Song, Z., Jiao, H., Song, Q., & Lin, H. (2012). The effect of group size and stocking density on the welfare and performance of hens housed in furnished cages during summer. Animal Welfare, 21(1), 41–49.

Gupta, S. K., Behera, K., Pradhan, C. R., Acharya, A. P., Sethy, K., Behera, D., Lone, S. A., & Shinde, K. P. (2016). Influence of stocking density on the performance, carcass characteristics, hemato-biochemical indices of Vanaraja chickens. Indian Journal of Animal Research, 51 (5), 939–943.

Hall, J. M. F., Witter, A. R., Racine, R. R., Berg, R. E., Podawiltz, A., Jones, H., & Mummert, M. E. (2014). Chronic psychological stress suppresses contact hypersensitivity: Potential roles of dysregulated cell trafficking and decreased IFN-γ production. Brain, Behavior, and Immunity, 36, 156–164.

Hedlund, L., Whittle, R., & Jensen, P. (2019). Effects of commercial hatchery processing on short- and long-term stress responses in laying hens. Scientific Reports, 9, 1–10.

Infante, M., Armani, A., Mammi, C., Fabbri, A., & Caprio, M. (2017). Impact of adrenal steroids on regulation of adipose tissue. Comprehensive Physiology, 7(4), 1425–1447.

Koronowicz, A.A., Banks, P., Szymczyk, B., Leszczyńska, T., Master, A., Piasna, E., Szczepański, W., Domagała, D., Kopeć, A., Piątkowska, E., & Laidler, P. (2016). Dietary conjugated linoleic acid affects blood parameters, liver morphology and expression of selected hepatic genes in laying hens. British Poultry Science, 57(5), 663–673.

Kraus, A., Zita, L., Krunt, O., Härtlová, H., & Chmelíková, E. (2021). Determination of selected biochemical parameters in blood serum and egg quality of Czech and Slovak native hens depending on the housing system and hen age. Poultry Science, 100 (2), 1142–1153.

Lara, L., & Rostagno, M. (2013). Impact of heat stress on poultry production. Animals, 3(2), 356–369.

Lin, H., Decuypere, E., & Buyse, J. (2006). Acute heat stress induces oxidative stress in broiler chickens. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 144(1), 11–17.

Mert, N., & Yildirim, B. (2016). Biochemical parameters and histopathological findings in the forced molt laying hens. Revista Brasileira de Ciência Avícola, 18(4), 711–718.

Mikami, T., Sumida, S., Ishibashi, Y., & Ohta, S. (2004). Endurance exercise training inhibits activity of plasma GOT and liver caspase-3 of rats exposed to stress by induction of heat shock protein 70. Journal of Applied Physiology, 96(5), 1776–1781.

Nwaigwe, C. U., Ihedioha, J. I., Shoyinka, S. V., & Nwaigwe, C. O. (2020). Evaluation of the hematological and clinical biochemical markers of stress in broiler chickens. Veterinary World, 13(10), 2294–2300.

Odihambo Mumma, J., Thaxton, J. P., Vizzier-Thaxton, Y., & Dodson, W. L. (2006). Physiological stress in laying hens. Poultry Science, 85(4), 761–769.

Olubodun, J., Zulkifli, I., Hair-Bejo, M., Kasim, A., & Soleimani, A. F. (2015). Physiological response of glutamine and glutamic acid supplemented broiler chickens to heat stress. European Poultry Science, 79, 1–12.

Pavlík, A., Pokludová, M., Zapletal, D., & Jelínek, P. (2007). Effects of housing systems on biochemical indicators of blood plasma in laying hens. Acta Veterinaria Brno, 76(3), 339–347.

Puvadolpirod, S., & Thaxton, J. P. (2000). Model of physiological stress in chickens 1. Response parameters. Poultry Science, 79(3), 363–369.

Rajman, M., Juráni, M., Lamošová, D., Máčajová, M., Sedlačková, M., Košťál, Ľ., Jezová, D., & Výboh, P. (2006). The effects of feed restriction on plasma biochemistry in growing meat type chickens (Gallus gallus). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 145(3), 363–371.

Ruiz-Jimenez, F., Gruber, E., Correa, M., & Crespo, R. (2021). Comparison of portable and conventional laboratory analyzers for biochemical tests in chickens. Poultry Science, 100(2), 746–754.

Sakhatsky, M., Osadcha, Y., & Kuchmistov, V. (2020). Reaction of the reproductive system of hens to the chronic stressor. Ukrainian Journal of Ecology, 10(4), 6–11.

Sandercock, D. A., Hunter, R. R., Mitchell, M. A., & Hocking, P. M. (2006). Thermoregulatory capacity and muscle membrane integrity are compromised in broilers compared with layers at the same age or body weight. British Poultry Science, 47(3), 322–329.

Shevchuk, M. O., Stoyanovskyy, V. G., & Kolomiiets, I. A. (2018). Technological stress in poultry. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 20(88), 63–68.

Sloan, E. K., Priceman, S. J., Cox, B. F., Yu, S., Pimentel, M. A., Tangkanangnukul, V., Arevalo, J. M., Morizono, K., Karanikolas, B. D., Wu, L., Sood, A. K., & Cole, S. W. (2010). The sympathetic nervous system induces a metastatic switch in primary breast cancer. Cancer Research, 70(18), 7042–7052.

Virden, W. S., & Kidd, M. T. (2009). Physiological stress in broilers: Ramifications on nutrient digestibility and responses. Journal of Applied Poultry Research, 18(2), 338–347.

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Published
2021-09-16
How to Cite
Sakhatsky, M. I., & Osadcha, Y. V. (2021). Clinical-biochemical status of hens due to changes of battery cages height location. Theoretical and Applied Veterinary Medicine, 9(3), 130-134. https://doi.org/10.32819/2021.93020