Species composition of microorganisms isolated from biomaterials of sick calves and cows

Keywords: bacteria; isolate; pathogen; sample; etiological structure; cattle

Abstract

Bacteriosis of cattle, the main cause of which is opportunistic microflora, cause significant damage to the livestock industry. Therefore, the study of the etiological structure of bacteriosis in cattle is relevant. During 2018, 2020–2021, 824 biomaterials’ samples of from 97 livestock farms in different regions of Ukraine were studied, including milk samples from patients with mastitis and vaginal lavages during cow endometritis, nasal mucus from calves with respiratory syndrome, feces from animals with diarrhea, exudate during inflammatory processes of the extremities. During bacteriological studies of biomaterials, 35 types of microorganisms were isolated. It should be noted that from 23.7% to 24.2% of Escherichia coli isolates were isolated; bacteria of the genus Proteus – from 10.8% to 16.7%. The important role of staphylococci in the occurrence of cattle bacteriosis has been proved, they have been isolated from 21.43% to 28.75%, while the proportion of Staphylococcus aureus isolation increased up to 21.6%. In the course of the biomaterials studies, up to 16.3% of enterococci were identified. According to our long-term research, simultaneous or sequential infection of animals with two or more pathogens has been established. Over the last year of observations (2021) the same trend was revealed towards an increase in the cases of two or more pathogens detection from one animals’ biomaterial with bacteriosis. If one is isolated in 31.11% of samples, while two – in 39.68%, three – in 18.41%.

Downloads

Download data is not yet available.

References

Ajshpur, O. Ye., Mushtuk, I. Yu., Sheremet, N. O., Berezovchuk, S. M., Gumenyuk, V. V., Yermolenko, O.M., & Derev’yanko, M. M. (2021). Vyvchennya antybiotykorezystentnosti enterokokiv. Vankomicynorezystentni shtamy enterokokiv [Study of antibiotic resistance of enterococci. Vancomycin-resistant strains of enterococci]. Veterynarna Biotekhnolohiia, 39, 9–17 (in Ukrainian).

Broens, E. M., Graat, E. A. M., Van Der Wolf, P. J., Van De Giessen, A. W., & De Jong, M. C. M. (2011). Prevalence and risk factor analysis of livestock associated MRSA-positive pig herds in The Netherlands. Preventive Veterinary Medicine, 102(1), 41–49.

Cuny, C., Nathaus, R., Layer, F., Strommenger, B., Altmann, D., & Witte, W. (2009). Nasal colonization of humans with methicillin-resistant Staphylococcus aureus (MRSA) CC398 with and without exposure to pigs. PLoS ONE, 4(8), e6800.

Fairbrother, J. M., & Gyles, C. L. (2012). Colibacillosis. In: Diseases of swine. Zimmerman J. J., Karriker L. A., Ramirez A., Schwartz K. J., Stevenson G. W. (Editors). Iowa State University Press. Ames, Iowa, USA. 10th edition, Chapter 53, 723–749.

Glebenjuk, V. V., & Telizhenko, K. V. (2015). Species belonging to mycobacteria isolated from animals in the Dnipropetrovsk region. Theoretical and Applied Veterinary Medicine, 3(1), 61–64.

Glebenyuk, V. V., Borovik, I. V., Kuchuk, T. V., & Litvinenko, O. O. (2018). Etiological structure of bacteriosis of animals in the Dnipropetrovsk region for 2014–2016. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 20(83), 260–263.

Gousia, P., Economou, V., Bozidis, P., & Papadopoulou, C. (2015). Vancomycin-resistance phenotypes, vancomycin-resistance genes, and resistance to antibiotics of enterococci isolated from food of animal origin. Foodborne Pathogens and Disease, 12(3), 214–220.

Gumenyuk, V. V. (2020). Vyvchennia antybiotykorezystentnosti klinichnykh shtamiv bakterii rodu proteus za bakterioziv vrkh [Study of antibiotic resistance of clinical strains of bacteria of the genus Proteus in cattle bacteriosis]. Aktualni Problemy Veterynarnoyi Biotexnologiyi ta Infekcijnoyi Patologiyi Tvaryn: materialy naukovo-praktychnoyi konferenciyi molodyx vchenyx, Kyiv (in Ukrainian).

Lytvyn, V. P., Olijnyk, L. V., Korniyenko, L. Ye., & Yarchuk, B. M. (2002). Faktorni khvoroby sil`s`kogospodars`kyx tvaryn [Factor diseases of farm animals]. Kyiv (in Ukrainian).

Perin, L. M., Miranda R. O., Todorov, S. D., Franco, B. D., & Nero, L. A. (2014). Virulence, antibiotic resistance and biogenic amines of bacteriocinogenic lactococci and enterococci isolated from goat milk. International Journal of Food Microbiology, 185(18), 121–126.

Pysarenko, V. F., Kovalenko, A. M., & Suvorova, V. N. (2014). Razrabotka preparata dlya profilaktiki i lecheniya krupnogo rogatogo skota pri razvitii infektsionnogo paltsevogo dermatita [Development of a drug for the prevention and treatment of cattle in the development of infectious finger dermatitis]. Vesnik Kurskoy Gosudarstvennoy Selskohozyaystvennoy Akademii, 6, 79–80 (in Russian).

Abstract views: 31
PDF Downloads: 21
Published
2021-12-30
How to Cite
Ayshpur, O., Mushtuk, I. Y., Gumeniuk, V. V., YermolenkoО. М., YermolenkoО. М., & Hlebeniuk, V. V. (2021). Species composition of microorganisms isolated from biomaterials of sick calves and cows. Theoretical and Applied Veterinary Medicine, 9(4), 173-181. https://doi.org/10.32819/2021.94027

Most read articles by the same author(s)