Morphogenesis of endochondral ossification centres of bones in racing pigeon chicks during the early postnatal period of ontogenesis

Keywords: organs of hemo- and lymphopoiesis; skeleton; bones; basic and additional centers of ossification; X-ray density; bone tissue; racing pigeons; chicks


Racing pigeons are potential carriers of zooanthroponic diseases, since they can travel considerable distances during their flight. The study of the formation features of the pigeon skeleton’s hematopoietic function on different levels of its structural organization with the determination of the relationship between the growth and development of hematopoietic components and the processes of osteohistogenesis is necessary to understand the immunity of pigeons, especially during the early stages of their development when young animals are in the greatest risk of disease, especially during processes of osteogenesis and haematopoiesis in chicks. The bones of the axial and peripheral skeleton of one-day-old, 5-, 10-, 15-, 20-, and 25-day-old pigeons (n = 5) were examined. The presence and degree of development of dia- and epiphyseal ossification centers (EOCs), their relative area (RA) and X-ray density in the organs of universal hematopoiesis were determined on radiographs made on an x-Ray-TW-102 x-ray machine with an Alpha 4600 receiver using the MultiVox Dicom Viewer program. It was established that in day-old pigeons the rudiments of the studied bones are completely built by cartilaginous tissue, have low radiographic density, and fuzzy contours on radiographs. On the 10th day of life, the axial skeleton of pigeon cubs has formed EOCs of head and a tubercle on the 5th rib (third «true rib»), while in the bones of the peripheral skeleton there is an enchondral EOC of the diaphysis. The spongy and compact bone structure (BS) of the bones were fuzzy. The body of the rib and the epiphyses of the limbs’ tubular bones were cartilaginous. The X-ray density of the humerus at this age remains unchanged, and the tibial-metatarsal increases by 1.5 times, reaching 12 HU. In 15-day-old chicks, the RA of the EOC in the skeleton was moderately increased due to an increase in the RA of previously formed EOCs at the age of 10 days, the appearance of individual EOCs in the body of 5th rib, and in the tubular bones of the limbs, the enchondral EOCs of the proximal and distal epiphyses. In the diaphysis of the extremities tubular bones, a strip-like compact BS was clearly distinguished, and in the epiphyses, a small-sized spongy BS. The X-ray density of the bones almost doubled, reaching 19–21 HU. In 20-day-old pigeons, the processes of osteohistogenesis in the skeleton were almost completed, in the studied bones, all the main and additional EOCs were well expressed, their RA increased sharply. X-ray density of bones did not change. For 25-day-old pigeons in the skeleton, there was a slight increase in RA of the EOC, a process of complete synostosis of the bones was characteristic. From the moment of the appearance of the EOC in 10-day-old chicks until they reach the age of 25 days, the bones of the axial skeleton and the skeleton of the limbs were formed by 95–100% of BS, and their X-ray density was increased almost 2.5 times, reaching 19–26 HU.


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Adler, C. (2006). Bones and bone tissue. Normal anatomy and histology. Bone Disseases. Springer-Verlag Berlin Heidelberg: New York, 1–30.

Araby, N., Soliman, S., Abdel Raheem, E., & Ahmed, Y. (2018). Morphogenesis of the Sternum in Quail Embryos. SVU-International Journal of Veterinary Sciences, 1(1), 16–24.

Bajmishev, H. B., Krishtoforova, B. V., Lemeshhenko, V. V., Hrustaleva, I. V. & Stegnej, Zh. G. (2013). Biologicheskie osnovy veterinarnoj neonatologii: monografija [Biological bases of veterinary neonatology: monograph]. Ric Sgsha, Samara (in Russian).

Baumgart, M., Szpinda, M. & Szpinda, A. (2012). New anatomical data on the growing C4 vertebra and its three ossification centers in human fetuses. Surgical and Radiologic Anatomy, 35(3), 191–203.

Bogomaz, А., Oliyar, A. & Shuleshko, O. (2016). Features development dynamics of bone morphometric parameters in pigeons of sports rocks in the early postnatal period of ontogenesis. Theoretical and Applied Veterinary Medicine, 4(2), 13–17 (in Ukranian).

Gavrilin, P. (2000). Strukturno-funkcionalni osoblyvosti organiv krovotvorennja teljat neonatalnogo i molochnogo periodiv. Extended abstract of doctor’s thesis. Kharkiv Zooveterinaty institute, Kharkiv (in Ukranian).

Gavrilin, P., Gavrilina, О., & Peretyatko, O. (2016). Structural and functional characteristics of bone marrow and components of the hematopoietic microenvironment in the centers of enchondral osteogenesis of the skeleton of newborn calves. Problems of Zooengineering and Veterinary Medicine, 32(2), 283–288 (in Ukranian).

Gavrilin, P., & Nykyforenko, O. (2005). Osoblyvosti formuvannya oseredkiv hemopoezu v kistkakh porosyat u neonatal’nyy ta molochnyy periody [Peculiarities of a forming the center of haemopoiesis in the bones of the piglets in early postnatal ontogenesis]. News of Dnipropetrovsk State Agrarian University, 2, 74–79 (in Ukranian).

Gavrilin, P., Oliyar, A., & Myrnyi, O. (2016). Peculiarities of morphogenesis of universal hematopoesis and immune protection in fetuses of domestic pig. The Animal Biology, 18(4), 30–34.

Grabchak, Zh. G. (2000). Morfofunkcionalnye osobennosti bedrennyh kostej neonatalnyh telyat [Morphofunctional features of the femurs of neonatal calves]. Veterinary Medicine: Іnterdepartmental Themed Science Compilation, 77, 106–111 (in Ukranian).

Kovtun, M. F., & Shatkovskaya, O. V. (2011). Vozniknovenie modeli ptencovogo razvitija u ptic: k probleme evoljucii ontogeneza [The origin of the model of chick development in birds: to the problem of evolution of ontogeny]. Vestnik Zoologii, 45(2), 161–171 (in Ukranian).

Krishtoforova, B. (2005). The priority directions of morphological researches into decision of rise newborn animals viability problem. News of Dnipropetrovsk State Agrarian University, 2, 190–192 (in Ukranian).

Krishtoforova, B. V., & Stegnej, Zh. G. (2013). Morfologichni osoblyvosti okremyh kistkovyh organiv kurchat [Morphological characteristics of individual bone of chicks]. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 15, 1(55), 1, 331–336 (in Ukranian).

Oliiar, A. V., & Lieshchova, M. A. (2019). Structural and functional organization of central organs of hematopoiesis and immune protection of piglets during the early postnatal period of ontogenesis. Theoretical and Applied Veterinary Medicine, 7(1), 8–13.

Oliyar, A., Lieshchova, M., & Logvinova, V. (2020). Morphogenesis of the centers of ossifi cation of bone organs of piglets in the early postnatal period of ontogenesis. Naukovij Vìsnik Veterinarnoï Medicini, 1(154), 113–120.

Rabson, A., Rojt, A., & Delvz, P. (2006). Osnovy medicinskoj immunologii [Fundamentals of Medical Immunology]. Moscow, Mir (in Russian).

Sapin, M. R., & Etingen, L. E. (1996). Immunnaya sistema cheloveka [Human immune system]. Moscow, Medicine (in Russian).

Shatkovska, O. V. (2001). Formuvannja skeleta kincivok v embriogenezi nagnizdnyh ta vyvodkovyh ptahiv. Extended abstract of candidate’s thesis. Schmalhausen Institute of Zoology of National Academy of Sciences of Ukraine, Кyiv (in Ukranian).

Skórzewska, A., Grzymisławska, M., Bruska, M., Łupicka, J., & Woźniak, W. (2013). Ossification of the vertebral column in human foetuses: histological and computed tomography studies. Folia Morphologica, 72(3), 230–238.

Snetkova, P. O. (2010). Morfofunktsionalni osoblyvosti kistkovoi systemy sobak neonatalnoho ta molochnoho periodiv. Extended abstract of candidate’s thesis. National university bioresource and environmental management of Ukraine, Кyiv (in Ukranian).

Sosonnyi, S. V. (2012). Zakonomirnosti morfohenezu krovotvornykh komponentiv skeleta plodiv velykoyi rohatoyi khudoby. Extended abstract of candidate’s thesis. Kharkiv State Zooveterinaty Academy, Kharkiv (in Ukranian).

Starck, J. M., & Ricklefs, R. E. (1998). Evolution within the altricial-precocial spectrum. New York: Oxford university press.

Szpinda, M., Baumgart, M., Szpinda, A., Woźniak, A., Małkowski, B., Wiśniewski, M., & Króliczewski, D. (2012). Cross-sectional study of the ossification center of the C1–S5 vertebral bodies. Surgical and Radiologic Anatomy, 35(5), 395–402.

Szpinda, M., Baumgart, M., Szpinda, A., Woźniak, A., Mila-Kierzenkowska, C., Dombek, M., & Grzybiak, M. (2013). Morphometric study of the T6 vertebra and its three ossification centers in the human fetus. Surgical and Radiologic Anatomy, 35(10), 901–916.

Szpinda, M., Baumgart, M., Szpinda, A., Woźniak, A., & Mila-Kierzenkowska, C. (2013). New patterns of the growing L3 vertebra and its 3 ossification centers in human fetuses – a CT, digital, and statistical study. Medical Science Monitor Basic Research, 19, 169–180.

Zinoviev, A. V. (2010). Sravnitel’naja anatomija, strukturnye preobrazovanija i adaptivnaja jevoljucija aparata dvunogoj lokomocii ptic [Comparative anatomy, structural transformations and adaptive evolution of the apparatus of bipedal locomotion in birds]. Moscow: KMK Scientific Publishing Association (in Russian).

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How to Cite
Oliyar, A. V., Bohomaz, A. A., Logvinova, V. V., Nikitina, M. O., & Lieshchova, M. A. (2022). Morphogenesis of endochondral ossification centres of bones in racing pigeon chicks during the early postnatal period of ontogenesis. Theoretical and Applied Veterinary Medicine, 9(4), 186-190.

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