COMPARISON OF METHODS EFFICIENCY FOR DETERMINATION OF SOMATIC CELLS COUNT IN GOAT MILK

  • N. Zazharska Dnipropetrovs’k State Agrarian and Economic University, Dnipro, Ukraine
  • L. Zharko Dnipropetrovs’k State Agrarian and Economic University, Dnipro, Ukraine
Keywords: goat milk, somatic cells count, milk films, fat, protein, freezing temperature, lactose

Abstract

The efficiency of determination of somatic cells count in goat milk by different methods was compared. Somatic cells in individual milk samples from 28 goats were analyzed by means of analyzer “Somatos”, “Soma Count Flow Cytometer” and the by counting of cells in milk films stained with pironin Y, at Romanovsky-Giemsa and May-Grunwald methods.When counting cells in milk films stained by any method there were not found the samples with somatic cells count to 100 ×103 cells / ml. According to the research by means of “Somatos” and “SomaCountFlowCytometer” most of milk films related to the level of – 101–500 ×103 cells / ml – 35.7 and 50% respectively. The largest part of stained milk films – by Romanovsky-Giemsa staining – 42.9% and by May-Grunwald staining – 39.3% belonged to the range of 1001-3000×103 cells / ml, whereas films, stained with pironin Y and methyl green – 35.7% – to a range of 501-1000×103 cells / ml.The greater number of cells in direct counting of somatic cells in goat milk films, stained by any method was determined than using appliances. The distribution ranges of somatic cells is similar between different methods of film staining, which once again proves the accuracy of the method of direct cell counting in milk films, though it is more labor-intensive methods than hardware. May-Grunwald staining is proposed for counting of somatic cells in goat milk films by Prescott and Breed method, because cytoplasm and nuclei of somatic cells are well stained, and cost much less than staining with pironin Y. While increasing of somatic cells count fat content was gradually raised, but significant difference was not found. Also there was a positive correlation between the somatic cells count and protein and lactose, but in the last group (somatic cell count of more than 3 million / ml), there was a sharp decrease. The opposite trend was observed regarding the freezing point of milk.It was found that while reducing of protein in goat milk, freezing temperature increased.

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Author Biographies

N. Zazharska, Dnipropetrovs’k State Agrarian and Economic University, Dnipro, Ukraine
к. вет. н.
L. Zharko, Dnipropetrovs’k State Agrarian and Economic University, Dnipro, Ukraine
студент

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How to Cite
Zazharska, N., & Zharko, L. (1). COMPARISON OF METHODS EFFICIENCY FOR DETERMINATION OF SOMATIC CELLS COUNT IN GOAT MILK. Theoretical and Applied Veterinary Medicine, 4(4), 29-35. Retrieved from https://bulletin-biosafety.com/index.php/journal/article/view/44
Section
Hygiene of Animals, Veterinary-Sanitary Expertise, Standardization, Quality and Safety of Agricultural Products