STUDY OF METABOLITES STRONGILOIDES PAPILLOSUS LARVAE ON THE FORMATION ERYTHROCYTES MICRONUCLEI IN PERIPHERAL BLOOD IN AN EXPERIMENT ON WHITE RATS

  • Ch. Shendrik Dnipropetrovsk State Agrarian and Economic University, Dnipro
  • L. Shendrik Dnipropetrovsk State Agrarian and Economic University, Dnipro
  • I. Shendrik Dnipropetrovsk State Agrarian and Economic University, Dnipro
Keywords: micronucleus test, mutagens, pathogenic influence, strongyloides larvae, strongyloidiasis

Abstract

Background. Helminthiases and now are pressing issue for livestock Ukraine. Strongyloidosis find dangerous highly pathogenic, well adapted to parasitism in the body different types of animals and humans and the continued existence in the environment. In the current research of special importance to the study of the relationship«parasite-host», namely the aspect mutagenic effects on the parasite hereditary apparatus of somatic and generative cells of the host. We know that excretory-secretory products of strongyloides, obviously helminths and other substances are unnatural for the host organism and in some way affect his physiological processes.To determine the level of such effects are cytogenetic methods, the use of which may be registered in mammalian chromosomal changes in the levels of genomic and genetic information. One of cytogenetic techniques of rapid assessment of the genetic dangers of such exposure are parasites micronucleus test.Objective. The aim was to investigate the mutagenic metabolites Strongyloides papillosus in the micronucleus test experimentally for strongyloidosis on white rats, the incidence of polihromatofil red blood cells in peripheral blood for the registration of genomic instability.Methods. Indicators micronucleus test was determined by 24 white male rats an average body weight of 130 g, which formed 4 groups of 6 animals each. Experimental animals the 1st, 2nd and 3rd groups infected, invasive larvae Strongyloides papillosus respectively, in the number – 5, 10 and 20 infective larvae per 1 g weight. Rats 4th group (intact) were controlled. A mixture of experimental concentrations of larvae in 2 % starch suspension in a volume of 0.2 ml was administered orally to animals with a rubber probe. Rats in the control group asked a pure starch slurry in the same volume. Determination of changes in red blood cells of rats was performed on the 7th, 14th, 21st and 42th days after infection larvae Strongyloides papillosus.To determine the changes in red blood cells, a drop of blood deposited onto a piece of glass, mixing it with a drop of 10 % sodium citrate and prepared smears. Ready smears, air dried, fixed in methanol and stained with Romanovsky. Using the microscope (90×10) counted the number of erythrocyte micronucleus normohromatofil 1000 cells each stroke. Micronuclei in blood cells was considered notable large formation with a diameter of 1/5-1/20 erythrocyte size. The experimental results are expressed in ppm (‰).Results. Analysis of samples of various series of experiments showed that the red blood cells of peripheral blood white rats of different groups of micronuclei were found with varying frequency to vary day experiment.Thus, the study of peripheral blood white rats in the control group showed that the number of red blood cells with micronuclei ranged from 1.0±0.48 to 1.3±0.33 in 1000 erythrocytes within 42 days of the study. In the blood of rats 1st experimental group was infected with invasive larvae of strogyloyides of 5 exp./g body weight at the 7-day study incidence of red blood cells with micronuclei slightly different from that of the rats in the control group and was 1.2±0.45 per 1000 erythrocytes. After 14 days the number of red blood cells with micronuclei increased to 2.2±0.52 ‰, and the 21 th day the number of micronuclei in erythrocytes significantly exceeded the figure of animals in the control group 2.6 times (p <0.05). After 42 days the experiment, the number of erythrocytes with micronuclei was 2.6±0.74 ‰, which probably did not differ from those parameters in rats in the control group.Conclusion. Lifetime excreta of larvae Strongyloides papillosus are mutagenic and can cause micronucleus formation of red blood cells in the peripheral blood of rats. At the 21st and the 42 nd day of the experiment, the number of red blood cells with micronuclei decreased in the peripheral blood of laboratory animals is probably a reducing of lifetime excreta larvae Strongyloides papillosus.

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Author Biographies

Ch. Shendrik, Dnipropetrovsk State Agrarian and Economic University, Dnipro
к. вет. н.
L. Shendrik, Dnipropetrovsk State Agrarian and Economic University, Dnipro
к. біол. н.
I. Shendrik, Dnipropetrovsk State Agrarian and Economic University, Dnipro
к. вет. н.

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How to Cite
Shendrik, C., Shendrik, L., & Shendrik, I. (1). STUDY OF METABOLITES STRONGILOIDES PAPILLOSUS LARVAE ON THE FORMATION ERYTHROCYTES MICRONUCLEI IN PERIPHERAL BLOOD IN AN EXPERIMENT ON WHITE RATS. Theoretical and Applied Veterinary Medicine, 4(4), 75-78. Retrieved from https://bulletin-biosafety.com/index.php/journal/article/view/53