SELECTION OF CRYOPROTECTIVE MEDIUM FOR FREEZE DRYING OF BRANCH STANDARD SAMPLE OF ANTI-RABIES IMMUNOGLOBULIN FROM RABBITS BLOOD SERUM

  • N. Mazur Institute of Veterinary Medicine of National Academy of Agrarian Sciences of Ukraine, Kyiv
  • I. Polupan Institute of Veterinary Medicine of National Academy of Agrarian Sciences of Ukraine, Kyiv
  • V. Nedosekov National University of Life and Environmental Sciences of Ukraine, Kyiv
Keywords: аnti-rabies immunoglobulin, branch standard sample, lyophilization, crioprotective medium

Abstract

Background. Despite the fact that much of immunodiagnostic biologicals in the one of final stages of production should be freeze drying, universal crioprotective medium for all biologicals is not exists. Thus, selection of optimal parameters of lyophilization cycle and protective medium is performed individually in each case. Objective. Selection of cryoprotective medium for rabies immunoglobulin from rabbits blood serum. Methods. We used sucrose, glucose, glycine, gelatin, glycerin and potato starch in different variations as stabilizing cryoprotective medium for anti-rabies immunoglobulin lyophilization. Lyophilization was performed using vacuum freeze drying installation Alpha 1–4, manufactured by Martin Christ GmbH (Germany). Anti-rabies activity of lyophilized sample was determined immediately after lyophilization. To assess the duration of storage lyophilized sample, we used method of “rapid aging” – storage in an incubator at 37°C for 4 weeks. Results. Established that protect medium number 2 (sucrose, glycine, gelatin) is most appropriate for lyophilization of anti-rabies immunoglobulin branch standard sample – a drop in anti-rabies potency after freeze drying was 12.8–18.4%, when using mediums number 1 (glucose, glycine, gelatin) and 3 (sucrose, glycine, potato starch) was at 16.2–23.2%, depending on evaluation method. Mediums number 4–6 had insufficient cryoprotective properties. For accelerated stability assessment of anti-rabies immunoglobulin lyophilized sample with protective medium number 1–3 we used “rapid aging” test. As a result the sample with protect medium number 1 anti-rabies potency decreased to 0.5 IU/ml and amounted to 9.1 IU/ml. Potency of sample with protect medium number 3 was at 9.6 IU/ml, and the sample number 2–9.9 IU/ml – decrease of anti-rabies potency level was 2.0 and 2.9 % respectively. Conclusion. Protect mediums number 2 (sucrose, glycine, gelatin) and 3(sucrose, glycine, potato starch) is most appropriate for lyophilization of branch standard sample of anti-rabies immunoglobulin.

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Author Biographies

N. Mazur, Institute of Veterinary Medicine of National Academy of Agrarian Sciences of Ukraine, Kyiv
аспірант
I. Polupan, Institute of Veterinary Medicine of National Academy of Agrarian Sciences of Ukraine, Kyiv
к. вет. наук
V. Nedosekov, National University of Life and Environmental Sciences of Ukraine, Kyiv
д. вет. наук

References

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REFERENCES

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How to Cite
Mazur, N., Polupan, I., & Nedosekov, V. (1). SELECTION OF CRYOPROTECTIVE MEDIUM FOR FREEZE DRYING OF BRANCH STANDARD SAMPLE OF ANTI-RABIES IMMUNOGLOBULIN FROM RABBITS BLOOD SERUM. Theoretical and Applied Veterinary Medicine, 5(1), 48-52. Retrieved from https://bulletin-biosafety.com/index.php/journal/article/view/78
Section
Microbiology, Virology and Biotechnology in Animal Industry and Veterinary Medicine